Meiotic recombination and spatial proximity in the etiology of the recurrent t(11;22)

Terry Ashley, Ann P. Gaeth, Hidehito Inagaki, Allen Seftel, Maimon M. Cohen, Lorinda K. Anderson, Hiroki Kurahashi, Beverly S. Emanuel

研究成果: ジャーナルへの寄稿学術論文査読

32 被引用数 (Scopus)

抄録

Although balanced translocations are among the most common human chromosomal aberrations, the constitutional t(11;22)(q23;q11) is the only known recurrent non-Robertsonian translocation. Evidence indicates that de novo formation of the t(11;22) occurs during meiosis. To test the hypothesis that spatial proximity of chromosomes 11 and 22 in meiotic prophase oocytes and spermatocytes plays a role in the rearrangement, the positions of the 11q23 and 22q11 translocation breakpoints were examined. Fluorescence in situ hybridization with use of DNA probes for these sites demonstrates that 11q23 is closer to 22q11 in meiosis than to a control at 6q26. Although chromosome 21p11, another control, often lies as close to 11q23 as does 22q11 during meiosis, chromosome 21 rarely rearranges with 11q23, and the DNA sequence of chromosome 21 appears to be less susceptible than 22q11 to double-strand breaks (DSBs). It has been suggested that the rearrangement recurs as a result of the palindromic AT-rich repeats at both 11q23 and 22q11, which extrude hairpin structures that are susceptible to DSBs. To determine whether the DSBs at these sites coincide with normal hotspots of meiotic recombination, immunocytochemical mapping of MLH1, a protein involved in crossing over, was employed. The results indicate that the translocation breakpoints do not coincide with recombination hotspots and therefore are unlikely to be the result of meiotic programmed DSBs, although MRE11 is likely to be involved. Previous analysis indicated that the DSBs appear to be repaired by a mechanism similar to nonhomologous end joining (NHEJ), although NHEJ is normally suppressed during meiosis. Taken together, these studies support the hypothesis that physical proximity between 11q23 and 22q11-but not typical meiotic recombinational activity in meiotic prophase-plays an important role in the generation of the constitutional t(11;22) rearrangement.

本文言語英語
ページ(範囲)524-538
ページ数15
ジャーナルAmerican Journal of Human Genetics
79
3
DOI
出版ステータス出版済み - 09-2006

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 遺伝学(臨床)

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