Metabolite profiling of plasma in patients with ossification of the posterior longitudinal ligament

Takashi Tsuji, Morio Matsumoto, Masaya Nakamura, Takeshi Miyamoto, Mitsuru Yagi, Nobuyuki Fujita, Eijiro Okada, Narihito Nagoshi, Osahiko Tsuji, Kota Watanabe

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Background: Metabolomics is one of the “omics” technologies, and is a comprehensive analysis of small molecule metabolites which include amino acid, nucleotides, carbohydrates and fatty acid. The purpose of the present study was to compare the differences of metabolite profiling between patients with ossification of the posterior longitudinal ligament (OPLL) and control subjects. Methods: We analyzed plasma metabolites in patients with cervical OPLL (n = 10) and in control subjects (n = 10). Ionic metabolites were analyzed using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) and lipophilic metabolites were analyzed using liquid chromatograph time-of-flight mass spectrometry (LC-TOFMS). Results: A total of 259 metabolites (144 metabolites in CE-TOFMS and 115 metabolites in LC-TOFMS) were detected. Among the 259 metabolites, six metabolites, namely acylcarnitine (AC) (14:0), palmitoylcarnitine, AC (18:2), fatty acid (FA) (24:2), thyroxine, thiaproline were significantly larger in OPLL group, even in analyzes excluding patients with diabetes mellitus and hyperlipidemia. Conclusions: We examined the metabolite profiling in patients with OPLL for the first time and detected six metabolites showing suggestive association with disease. These results of the present study could lead to new insights into clarifying the molecular pathomechanisms of OPLL.

本文言語English
ページ(範囲)878-883
ページ数6
ジャーナルJournal of Orthopaedic Science
23
6
DOI
出版ステータスPublished - 11-2018

All Science Journal Classification (ASJC) codes

  • 外科
  • 整形外科およびスポーツ医学

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