Microinjected F-actin into dividing newt eggs moves toward the next cleavage furrow together with Ca2+ stores with inositol 1,4,5-trisphospnate receptor in a microtubule- and microtubule motor- dependent manner

Fuyuki Mitsuyama, Yoshio Futatsugi, Masato Okuya, Kostadin Karagiozov, Yoko Kato, Tetsuo Kanno, Hirotosni Sano, Tadashi Koide, Tsuyosi Sawai

研究成果: Article査読

2 被引用数 (Scopus)

抄録

It has been reported that F-actin is transported to the presumptive cleavage furrow along the cortex during anaphase-cytokinesis, an event termed cortical actin flow in animal cultured cells. The motor source has remained unknown. We reported that Ca2+ stores with IP3 receptor (IP3R) was re-distributed from the polar cortex during metaphase to the presumptive cleavage furrow just before the onset of furrowing, and that Ca2+ stores with IP3R microinjected into dividing newt eggs moved toward the presumptive cleavage furrow during anaphase-cytokinesis in a microtubule-de- pendent manner, and that Ca2+ store-enriched microsome fractions induced the cleavage furrow as the putative cleavage stimulus. Because the distribution of F-actin and Ca2+ stores with IP3R during metaphase to cytokinesis is similar, we considered that this cortical actin flow may be powered by transportation of Ca2+ stores with D?3R. Purified F-actin labeled with phalloidin-rhodamine was microinjected into the dividing newt eggs and the eggs observed under a confocal microscope. We found that the microinjected F-actin moved linearly toward the next cleavage furrow and that this movement was blocked by nocodazole, microtubule-depolarizing agent and AMP-PNP, a blocking agent of microtubule motors. Co-microinjected rhodamine-labeled F-actin and sacro / endoplasmic reticulum Ca2+-ATPase (SERCA)-GFP-labeled Ca2+ stores with IP3R co-moved and co-accumulated to the next cleavage furrow. These results strongly suggest that Ca2+ stores with IP 3R, which is transferred by microtubule-based motility as cleavage stimulus, act as an F-actin translocator.

本文言語English
ページ(範囲)143-151
ページ数9
ジャーナルItalian Journal of Anatomy and Embryology
113
3
出版ステータスPublished - 01-07-2008

All Science Journal Classification (ASJC) codes

  • 解剖学
  • 胎生学

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「Microinjected F-actin into dividing newt eggs moves toward the next cleavage furrow together with Ca<sup>2+</sup> stores with inositol 1,4,5-trisphospnate receptor in a microtubule- and microtubule motor- dependent manner」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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