MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells

Koichiro Minami, Kohei Taniguchi, Nobuhiko Sugito, Yuki Kuranaga, Teruo Inamoto, Kiyoshi Takahara, Tomoaki Takai, Yuki Yoshikawa, Satoshi Kiyama, Yukihiro Akao, Haruhito Azuma

研究成果: Article

30 引用 (Scopus)

抜粋

The Warburg effect is a well-known feature in cancer-specific metabolism. We previously reported on the role of microRNA (miR)-145 as a tumor-suppressor in human bladder cancer (BC) cells. In this study, we reveal that miR-145 decreases the Warburg effect by silencing KLF4 in BC cells. The expression levels of miR-145 were significantly lower in clinical BC samples and BC cell lines compared to those in normal tissues and HUC cells. Luciferase assay results showed that miR-145 directly bound to 3'UTR of KLF4, which was shown to be overexpressed in the clinical BC samples using Western blot analysis and immunohistochemistry. Remarkable growth inhibition and apoptosis were induced by the ectopic expression of miR-145 or by the gene silencing of KLF4 (siR-KLF4). Also, Warburg effect-related genes such as PTBP1/PKMs were regulated by the transfection of BC cells with miR-145 or siR-KLF4. These results thus indicate that the miR-145/KLF4/PTBP1/PKMs axis is one of the critical pathways that maintain the Warburg effect in BC carcinogenesis. MiR-145 perturbed the Warburg effect by suppressing the KLF4/PTBP1/PKMs pathway in BC cells, resulting in significant cell growth inhibition.

元の言語English
ページ(範囲)33064-33077
ページ数14
ジャーナルOncotarget
8
発行部数20
DOI
出版物ステータスPublished - 01-01-2017

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Oncology

これを引用

Minami, K., Taniguchi, K., Sugito, N., Kuranaga, Y., Inamoto, T., Takahara, K., Takai, T., Yoshikawa, Y., Kiyama, S., Akao, Y., & Azuma, H. (2017). MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget, 8(20), 33064-33077. https://doi.org/10.18632/oncotarget.16524