Modulation of pacemaker activity of sinoatrial node cells by electrical load imposed by an atrial cell model

Eiichi Watanabe, H. Honjo, T. Anno, M. R. Boyett, I. Kodama, J. Toyama

研究成果: Article

41 引用 (Scopus)

抜粋

To investigate the electrotonic modulation of sinoatrial (SA) node pacemaker activity by atrial muscle, single or multiple (2-7) SA node cells isolated from rabbit hearts were connected to a membrane model [resistance- capacitance (R-C) circuit] of an atrial cell through an external circuit that mimics the gap junctional conductance (G(c)) between cells. When G(c) was 0 nS (uncoupled conditions), all the preparations generated regular and stable spontaneous action potentials with a mean cycle length (SCL) of 263 ± 45 ms (±SD, n = 35). Step increases of G(c) were associated with a progressive prolongation of SCL. At sufficiently high values of G(c), the spontaneous activity became irregular and finally stopped. We defined the threshold G(c) causing an appreciable SCL irregularity as the minimum G(c) at which the ratio of SD to mean of SCL was > 0.3. The threshold G(c) for a single SA node cell was calculated to be 0.58 nS. In the presence of acetylcholine (ACh; 0.05-0.2 μM), the coupling-induced inhibition of spontaneous activity was greatly increased, and the threshold G(c) for a single SA node cell was decreased in a concentration-dependent manner. These findings show that the pacemaker activity of SA node cells is easily inhibited when the cells are coupled to a passive atrial cell model and the inhibition is amplified by ACh. Computer simulation using a modified Oxsoft HEART model indicates that the passive atrial cell model acts as a current sink, imposing a substantial outward current on the SA node cell, and ACh amplifies the effect by activating an additional outward current.

元の言語English
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
269
発行部数5 38-5
出版物ステータスPublished - 01-01-1995
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

フィンガープリント Modulation of pacemaker activity of sinoatrial node cells by electrical load imposed by an atrial cell model' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用