抄録
Chromosome 3p is frequently deleted in various cancers including examples in the lung. A novel gene, termed FHIT, was recently isolated from the fragile site at 3p14.2, with aberrant transcripts being reported in lung cancer tumor specimens. To avoid overlooking tumor-specific altered transcripts due to contaminating normal cells in primary tumors, FHIT alterations were examined in 41 lung cancer cell lines in the present study. Lack of detectable expression or exclusive expression of aberrantly spliced transcripts, often accompanied by intragenic homozygous deletions, were observed in 7 of 24 non-small cell lung cancers (29%) but in 0 of 17 small cell lung cancers (0%). Extensive reverse transcription-PCR-single-strand conformation polymorphism analysis revealed polymorphisms and alternative splicing but failed to identify point mutations. These results suggest distinct mechanisms for FHIT alterations in lung tumorigenesis and that further studies of this interesting gene are warranted.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 5579-5582 |
| ページ数 | 4 |
| ジャーナル | Cancer Research |
| 巻 | 56 |
| 発行部数 | 24 |
| 出版物ステータス | Published - 15-12-1996 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
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Molecular analysis of the FHIT gene at 3p14.2 in lung cancer cell lines. / Yanagisawa, Kiyoshi; Kondo, Masashi; Osada, Hirotaka; Uchida, Kosaku; Takagi, Kenzo; Masuda, Akira; Takahashi, Toshitada; Takahashi, Takashi.
：: Cancer Research, 巻 56, 番号 24, 15.12.1996, p. 5579-5582.研究成果: Article
TY - JOUR
T1 - Molecular analysis of the FHIT gene at 3p14.2 in lung cancer cell lines
AU - Yanagisawa, Kiyoshi
AU - Kondo, Masashi
AU - Osada, Hirotaka
AU - Uchida, Kosaku
AU - Takagi, Kenzo
AU - Masuda, Akira
AU - Takahashi, Toshitada
AU - Takahashi, Takashi
PY - 1996/12/15
Y1 - 1996/12/15
N2 - Chromosome 3p is frequently deleted in various cancers including examples in the lung. A novel gene, termed FHIT, was recently isolated from the fragile site at 3p14.2, with aberrant transcripts being reported in lung cancer tumor specimens. To avoid overlooking tumor-specific altered transcripts due to contaminating normal cells in primary tumors, FHIT alterations were examined in 41 lung cancer cell lines in the present study. Lack of detectable expression or exclusive expression of aberrantly spliced transcripts, often accompanied by intragenic homozygous deletions, were observed in 7 of 24 non-small cell lung cancers (29%) but in 0 of 17 small cell lung cancers (0%). Extensive reverse transcription-PCR-single-strand conformation polymorphism analysis revealed polymorphisms and alternative splicing but failed to identify point mutations. These results suggest distinct mechanisms for FHIT alterations in lung tumorigenesis and that further studies of this interesting gene are warranted.
AB - Chromosome 3p is frequently deleted in various cancers including examples in the lung. A novel gene, termed FHIT, was recently isolated from the fragile site at 3p14.2, with aberrant transcripts being reported in lung cancer tumor specimens. To avoid overlooking tumor-specific altered transcripts due to contaminating normal cells in primary tumors, FHIT alterations were examined in 41 lung cancer cell lines in the present study. Lack of detectable expression or exclusive expression of aberrantly spliced transcripts, often accompanied by intragenic homozygous deletions, were observed in 7 of 24 non-small cell lung cancers (29%) but in 0 of 17 small cell lung cancers (0%). Extensive reverse transcription-PCR-single-strand conformation polymorphism analysis revealed polymorphisms and alternative splicing but failed to identify point mutations. These results suggest distinct mechanisms for FHIT alterations in lung tumorigenesis and that further studies of this interesting gene are warranted.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029731629&partnerID=8YFLogxK
M3 - Article
C2 - 8971157
AN - SCOPUS:0029731629
VL - 56
SP - 5579
EP - 5582
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 24
ER -