Molecular characterization of a functional cDNA for rat substance P receptor

Y. Yokota, Y. Sasai, K. Tanaka, T. Fujiwara, K. Tsuchida, R. Shigemoto, A. Kakizuka, H. Ohkubo, S. Nakanishi

研究成果: Article

389 引用 (Scopus)

抄録

This paper describes the amino acid sequence of the rat substance P receptor and its comparison with that of the rat substance K receptor on the basis of molecular cloning and sequence analysis. From a rat brain cDNA library constructed with an RNA expression vector, we identified a cDNA mixture containing a functional substance P receptor cDNA by examining electrophysiologically a receptor expression following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A receptor cDNA clone was then isolated by cross-hybridization with the bovine substance K receptor DNA. The clone was confirmed by selective binding of substance P to the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence (407 amino acid residues) possesses seven putative membrane spanning domains and shows a sequence similarity to the members of G-protein-coupled receptors. The rat substance P and substance K receptor are very similar in both size and amino acid sequences, particularly in the putative transmembrane similarity is in marked contrast to the sequence divergence in the amino- and carboxyl-terminal regions and the third cytoplasmic loop. The observed sequence similarytity and divergence would thus contribute to the expression of similar but pharmacological regions and the first and second cytoplasmic loops. This distinguishable activities of the two tachykinin receptors.

元の言語English
ページ(範囲)17649-17652
ページ数4
ジャーナルJournal of Biological Chemistry
264
発行部数30
出版物ステータスPublished - 01-01-1989

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Neurokinin-1 Receptors
Neurokinin-2 Receptors
Rats
Complementary DNA
Amino Acids
Amino Acid Sequence
Substance P
Clone Cells
Tachykinin Receptors
Cloning
COS Cells
Molecular Cloning
G-Protein-Coupled Receptors
Xenopus
Gene Library
Oocytes
Sequence Analysis
Brain
Pharmacology
RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Yokota, Y., Sasai, Y., Tanaka, K., Fujiwara, T., Tsuchida, K., Shigemoto, R., ... Nakanishi, S. (1989). Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry, 264(30), 17649-17652.
Yokota, Y. ; Sasai, Y. ; Tanaka, K. ; Fujiwara, T. ; Tsuchida, K. ; Shigemoto, R. ; Kakizuka, A. ; Ohkubo, H. ; Nakanishi, S. / Molecular characterization of a functional cDNA for rat substance P receptor. :: Journal of Biological Chemistry. 1989 ; 巻 264, 番号 30. pp. 17649-17652.
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abstract = "This paper describes the amino acid sequence of the rat substance P receptor and its comparison with that of the rat substance K receptor on the basis of molecular cloning and sequence analysis. From a rat brain cDNA library constructed with an RNA expression vector, we identified a cDNA mixture containing a functional substance P receptor cDNA by examining electrophysiologically a receptor expression following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A receptor cDNA clone was then isolated by cross-hybridization with the bovine substance K receptor DNA. The clone was confirmed by selective binding of substance P to the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence (407 amino acid residues) possesses seven putative membrane spanning domains and shows a sequence similarity to the members of G-protein-coupled receptors. The rat substance P and substance K receptor are very similar in both size and amino acid sequences, particularly in the putative transmembrane similarity is in marked contrast to the sequence divergence in the amino- and carboxyl-terminal regions and the third cytoplasmic loop. The observed sequence similarytity and divergence would thus contribute to the expression of similar but pharmacological regions and the first and second cytoplasmic loops. This distinguishable activities of the two tachykinin receptors.",
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Yokota, Y, Sasai, Y, Tanaka, K, Fujiwara, T, Tsuchida, K, Shigemoto, R, Kakizuka, A, Ohkubo, H & Nakanishi, S 1989, 'Molecular characterization of a functional cDNA for rat substance P receptor', Journal of Biological Chemistry, 巻. 264, 番号 30, pp. 17649-17652.

Molecular characterization of a functional cDNA for rat substance P receptor. / Yokota, Y.; Sasai, Y.; Tanaka, K.; Fujiwara, T.; Tsuchida, K.; Shigemoto, R.; Kakizuka, A.; Ohkubo, H.; Nakanishi, S.

:: Journal of Biological Chemistry, 巻 264, 番号 30, 01.01.1989, p. 17649-17652.

研究成果: Article

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N2 - This paper describes the amino acid sequence of the rat substance P receptor and its comparison with that of the rat substance K receptor on the basis of molecular cloning and sequence analysis. From a rat brain cDNA library constructed with an RNA expression vector, we identified a cDNA mixture containing a functional substance P receptor cDNA by examining electrophysiologically a receptor expression following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A receptor cDNA clone was then isolated by cross-hybridization with the bovine substance K receptor DNA. The clone was confirmed by selective binding of substance P to the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence (407 amino acid residues) possesses seven putative membrane spanning domains and shows a sequence similarity to the members of G-protein-coupled receptors. The rat substance P and substance K receptor are very similar in both size and amino acid sequences, particularly in the putative transmembrane similarity is in marked contrast to the sequence divergence in the amino- and carboxyl-terminal regions and the third cytoplasmic loop. The observed sequence similarytity and divergence would thus contribute to the expression of similar but pharmacological regions and the first and second cytoplasmic loops. This distinguishable activities of the two tachykinin receptors.

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Yokota Y, Sasai Y, Tanaka K, Fujiwara T, Tsuchida K, Shigemoto R その他. Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry. 1989 1 1;264(30):17649-17652.