TY - JOUR
T1 - Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens
AU - Shahcheraghi, Fereshteh
AU - Minato, Yusuke
AU - Chen, Jing
AU - Mizushima, Tohru
AU - Ogawa, Wakano
AU - Kuroda, Teruo
AU - Tsuchiya, Tomofusa
PY - 2007/4
Y1 - 2007/4
N2 - We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.
AB - We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.
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U2 - 10.1248/bpb.30.798
DO - 10.1248/bpb.30.798
M3 - Article
C2 - 17409524
AN - SCOPUS:34147127625
SN - 0918-6158
VL - 30
SP - 798
EP - 800
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 4
ER -