TY - JOUR
T1 - Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver
AU - Sakurada, Kazuhiro
AU - Uchida, Kazuhisa
AU - Yamaguchi, Kazuo
AU - Aisaka, Kazuo
AU - Ito, Seiga
AU - Ohmori, Toshihiko
AU - Takeyama, Yoshifumi
AU - Ueda, Takashi
AU - Hori, Yuichi
AU - Ohyanagi, Harumasa
AU - Saitoh, Yoichi
AU - Kaibuchi, Kozo
AU - Takai, Yoshimi
N1 - Funding Information:
1 The investigation in the Department of Surgery (1st Division), Kobe University School of Medicine, was supported by a grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (1990). The investigation in the Department of Biochemistry, Kobe University School of Medicine, was supported by grants-in-aid for Scientific Research and Cancer Research from the Ministry Education, Science and Culture, Japan (1990), a grant-in-aid for Abnormalities in Hormone Recep-tor, Metabolic Disease from the Ministry of Health and Welfare, Japan (1990), and by grants from the Yamanouchi Foundation for
PY - 1991/6/28
Y1 - 1991/6/28
N2 - We have isolated cDNA clones from a rat liver cDNA library that encode a ras p21-like small GTP-binding protein (24KG) which was purified from the microsomes-Golgi complex fraction of the rat liver. The cloning was accomplished using polymerase chain reaction amplified with a set of oligonucleotide primers which were designed from the partial amino acid sequences for 24KG. The cDNA contained an open reading frame encoding a 216 amino acid protein with a calculated Mr weight of 24,397. This Mr weight was similar to that of the purified 24KG estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sequence analysis of 24KG revealed that a 24KG cDNA is the rat counterpart of a rab11 cDNA cloned from a Madin-Darby canine kidney cell cDNA library. The 1.0-kilobase 24KG mRNA corresponding to the isolated cDNA was also detected in various rat tissues, such as brain, testis, spleen, and heart.
AB - We have isolated cDNA clones from a rat liver cDNA library that encode a ras p21-like small GTP-binding protein (24KG) which was purified from the microsomes-Golgi complex fraction of the rat liver. The cloning was accomplished using polymerase chain reaction amplified with a set of oligonucleotide primers which were designed from the partial amino acid sequences for 24KG. The cDNA contained an open reading frame encoding a 216 amino acid protein with a calculated Mr weight of 24,397. This Mr weight was similar to that of the purified 24KG estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sequence analysis of 24KG revealed that a 24KG cDNA is the rat counterpart of a rab11 cDNA cloned from a Madin-Darby canine kidney cell cDNA library. The 1.0-kilobase 24KG mRNA corresponding to the isolated cDNA was also detected in various rat tissues, such as brain, testis, spleen, and heart.
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U2 - 10.1016/0006-291X(91)90672-T
DO - 10.1016/0006-291X(91)90672-T
M3 - Article
C2 - 1711847
AN - SCOPUS:0025781448
SN - 0006-291X
VL - 177
SP - 1224
EP - 1232
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -