Molecular cloning of a mouse 47‐kDa heat‐shock protein (HSP47), a collagen‐binding stress protein, and its expression during the differentiation of F9 teratocarcinoma cells

Hajime TAKECHI, Kazunori HIRAYOSHI, Akira NAKAI, Hitoshi KUDO, Shinsuke SAGA, Kazuhiro NAGATA

研究成果: Article査読

93 被引用数 (Scopus)

抄録

A 47‐kDa heat‐shock protein (HSP47) is a major collagen‐binding stress protein residing in the endoplasmic reticulum, and is assumed to be a molecular chaperone specific to collagen. Two‐dimensional gel electrophoresis and immunoprecipitation studies showed that the expression of HSP47 was significantly induced during the differentiation of mouse teratocarcinoma F9 cells by treatment with retinoic acid alone or with retinoic acid and dibutyryladenosine 3′,5′‐phosphate. The induction of type‐IV collagen was also observed during F9‐cell differentiation. For further analysis, we cloned cDNA encoding mouse HSP47 from a cDNA library of BALB/c 3T3 cells and performed Northern‐blot analysis. The cDNA contained a signal sequence at the N‐terminus and an endoplasmic‐reticulum‐retention signal, RDEL, at the C‐terminus. An homology search revealed that mouse HSP47, as well as chick HSP47, belonged to the serine protease inhibitor superfamily. While chick HSP47 mRNA was 4.5 kb with a long (2‐kb) 3′ untranslated region, mouse and human HSP47 mRNA were 2.5 kb, with a 0.8‐kb 3′ untranslated region. Northern‐blot analysis revealed that the concurrent induction of HSP47 and type‐IV collagen during F9‐cell differentiation, and the transient induction of HSP47 after heat shock was regulated at the level of mRNA accumulation. These results suggested that HSP47 was closely related to collagens in terms of its expression as well as in its functional relevance.

本文言語English
ページ(範囲)323-329
ページ数7
ジャーナルEuropean Journal of Biochemistry
206
2
DOI
出版ステータスPublished - 06-1992

All Science Journal Classification (ASJC) codes

  • Biochemistry

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