Molecular Epidemiology of Enterobacter cloacae Complex Isolates with Reduced Carbapenem Susceptibility Recovered by Blood Culture

Jayathilake Sarangi, Nao Matsuo, Rina Nonogaki, Michiko Hayashi, Kumiko Kawamura, Masahiro Suzuki, Wanchun Jin, Kiyoko Tamai, Miho Ogawa, Jun Ichi Wachino, Kouji Kimura, Tetsuya Yagi, Yoshichika Arakawa

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The Enterobacter cloacae complex (ECC) is one of the most common causes of bacteremia and leads to poor clinical outcomes. The aim of this study was to clarify the antimicrobial susceptibility profiles and genetic backgrounds of non-carbapenemase-producing reduced-carbapenem-susceptible (RCS) ECC blood isolates in Japan using agar dilution antimicrobial susceptibility testing, whole-genome sequencing, and quantitative polymerase chain reaction for ampC, ompC, and ompF transcripts. Forty-two ECC blood isolates were categorized into RCS and carbapenem-susceptible groups based on the minimum inhibitory concentration of imipenem. The RCS ECC blood isolates belonged to distinct species and sequence types and produced varying class C β-lactamases. The E. roggenkampii, E. asburiae, and E. bugandensis isolates belonged only to the RCS group. Some E. hormaechei ssp. steigerwaltii isolates from the RCS group exhibited AmpC overexpression caused by amino acid substitutions in AmpD and AmpR along with ompF downregulation. These findings suggest that non-carbapenemase-producing RCS ECC blood isolates are genetically diverse.

本文言語English
ページ(範囲)41-48
ページ数8
ジャーナルJapanese journal of infectious diseases
75
1
DOI
出版ステータスPublished - 2022

All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 感染症

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