Molecular mechanisms regulating dissociation of cell-cell junction of epithelial cells by oxidative stress

Junko Inumaru, Osamu Nagano, Eri Takahashi, Takatsugu Ishimoto, Satoshi Nakamura, Yoshimi Suzuki, Shin Ichiro Niwa, Kazuo Umezawa, Hidenobu Tanihara, Hideyuki Saya

研究成果: ジャーナルへの寄稿学術論文査読

64 被引用数 (Scopus)


Oxidative stress is regarded as a causative factor in aging and various degenerative diseases. Here, we show the mechanism by which oxidative stress induces disruption of cell-cell junctions using retinal pigment epithelial cells. We demonstrated that reactive oxygen species (ROS)-mediated activation of Src kinase increases the tyrosine phosphorylation state of p120-catenin and rapidly triggers translocation of p120-catenin and internalization of N-cadherin from the cell-cell adhesion sites to an early endosomal compartment. Endosomal accumulation of p120-catenin resulted in stress fiber formation and cell-cell dissociation through the activation of Rho/Rho kinase pathway. However, these cytoskeletal remodeling and cell-cell dissociation induced by oxidative stress were transient, due to the activation of nuclear factor-κB (NF-κB) and the expression of manganese superoxide dismutase (Mn-SOD). Using the NF-κB specific inhibitor DHMEQ, we found that NF-κB is part of a negative feedback loop to control intracellular ROS levels. Finally, we demonstrated that H2 O2 treatment alone does not induce the epithelial mesenchymal transition (EMT) in retinal pigment epithelial cells, which can be induced by TNF-α treatment. These findings suggest that oxidative stress is a crucial factor to induce the cell-cell dissociation, an initial step of EMT, but does not provide sufficient signals to establish and to maintain the EMT.

ジャーナルGenes to Cells
出版ステータス出版済み - 15-06-2009

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 細胞生物学


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