Molecular nature and possible presence of a membranous glycan-phosphatidylinositol anchor of CA125 antigen

Atsuo Nagata, Norio Hirota, Takao Sakai, Masao Fujimoto, Tsugikazu Komoda

研究成果: Article査読

28 被引用数 (Scopus)

抄録

The molecular nature and possible presence of a glycan-phosphatidylinositol anchor (GPI-anchor) in CA125 molecules was investigated. Serial lectin affinity chromatography and N-or O-glycanase treatment to reduce antigenicity showed that CA125 contained certain N-and O-glycosylated sugar chains in the molecule, like a glycoprotein. CA125 released from ovarian cancer tissues increased time-dependently following phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, concomitant with the release of tissue-unspecific alkaline phosphatase. Western blotting of CA125 treated by PI-PLC showed a single band of 90 kD instead of the 162-and 76-kD bands of the native antigen. Further, ovarian cancer tissues subjected to PI-PLC treatment lost the immunohistochemical localization of CA125 with OC125 antibody. Consequently, it is strongly suggested that CA125 is a glycoprotein that has both N-and O-linked sugar chains and a membranous GPI-anchoring moiety, and further, that its 90-kD form is the antigen without the GPI-anchor.

本文言語English
ページ(範囲)279-286
ページ数8
ジャーナルTumor Biology
12
5
DOI
出版ステータスPublished - 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 癌研究

フィンガープリント

「Molecular nature and possible presence of a membranous glycan-phosphatidylinositol anchor of CA125 antigen」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル