TY - JOUR
T1 - Monitoring four herpesviruses in unrelated cord blood transplantation
AU - Tanaka, N.
AU - Kimura, H.
AU - Hoshino, Y.
AU - Kato, K.
AU - Yoshikawa, T.
AU - Asano, Y.
AU - Horibe, K.
AU - Kojima, S.
AU - Morishima, T.
N1 - Funding Information:
We thank Prof K Watanabe, Department of Pediatrics, Nagoya University School of Medicine, for helpful advice, and Prof K Yamanishi, Department of Microbiology, Osaka University School of Medicine, for a gift of PSTY05. This work was supported by a grant from Japan Society for the Promotion of Science (JSPS-RFTF97L00703) and also by a grant from Ministry of Health and Welfare, Health Science Research Grants, Research on Immunology, Allergy and Organ Transplantation (H11-Menneki-006).
PY - 2000
Y1 - 2000
N2 - Cord blood transplantation, which has lower risk of graft-versus-host disease than bone marrow transplantation, might have higher risk of infections. A system to quantify four herpesviruses, CMV, human herpesvirus 6 (HHV6), EBV, varicella-zoster virus using the real-time PCR assay was established and applied for prospective viral load monitoring in three recipients undergoing cord blood transplantation. CMV and HHV6 were detected in peripheral blood from all three recipients, while EBV was detected in two. Varicellazoster virus was not detected at all. At the peak of HHV6 or CMV, each patient showed virus-related symptoms. During the pre-transplant period, CMV DNA was detected in two recipients who later developed CMV-related diseases. These observations indicate that our system is not only useful for managing herpesviruses infections in transplant recipients, but also a powerful method for clarifying the relationships between the viral load and clinical symptoms.
AB - Cord blood transplantation, which has lower risk of graft-versus-host disease than bone marrow transplantation, might have higher risk of infections. A system to quantify four herpesviruses, CMV, human herpesvirus 6 (HHV6), EBV, varicella-zoster virus using the real-time PCR assay was established and applied for prospective viral load monitoring in three recipients undergoing cord blood transplantation. CMV and HHV6 were detected in peripheral blood from all three recipients, while EBV was detected in two. Varicellazoster virus was not detected at all. At the peak of HHV6 or CMV, each patient showed virus-related symptoms. During the pre-transplant period, CMV DNA was detected in two recipients who later developed CMV-related diseases. These observations indicate that our system is not only useful for managing herpesviruses infections in transplant recipients, but also a powerful method for clarifying the relationships between the viral load and clinical symptoms.
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U2 - 10.1038/sj.bmt.1702710
DO - 10.1038/sj.bmt.1702710
M3 - Article
C2 - 11149730
AN - SCOPUS:0033665561
VL - 26
SP - 1193
EP - 1197
JO - Bone Marrow Transplantation
JF - Bone Marrow Transplantation
SN - 0268-3369
IS - 11
ER -