Multiple roles of vertebrate REV genes in DNA repair and recombination

Takashi Okada, Eiichiro Sonoda, Michio Yoshimura, Yoshiaki Kawano, Hideyuki Saya, Masaoki Kohzaki, Shunichi Takeda

研究成果: Article査読

91 被引用数 (Scopus)

抄録

In yeast, Rev1, Rev3, and Rev7 are involved in translesion synthesis over various kinds of DNA damage and spontaneous and UV-induced mutagenesis. Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40. REV1 -/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants. Surprisingly, the triple-knockout cells showed a suppressed level of sister chromatid exchanges (SCEs), which may reflect postreplication repair events mediated by homologous recombination, while each single mutant showed an elevated SCE level. Furthermore, REV1-/- cells as well as triple mutants showed a decreased level of immunoglobulin gene conversion, suggesting participation of Rev1 in a recombination-based pathway. The present study gives us a new insight into cooperative function of three Rev molecules and the Polζ (Rev3-Rev7)-independent role of Rev1 in vertebrate cells.

本文言語English
ページ(範囲)6103-6111
ページ数9
ジャーナルMolecular and Cellular Biology
25
14
DOI
出版ステータスPublished - 07-2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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