Myosin II activation promotes neurite retraction during the action of Rho and Rho-kinase

Mutsuki Amano, Kazuyasu Chihara, Nao Nakamura, Yuko Fukata, Takeo Yano, Masao Shibata, Mitsuo Ikebe, Kozo Kaibuchi

研究成果: Article査読

218 被引用数 (Scopus)

抄録

Background: The Rho small GTPase regulates myosin II activity through the phosphorylation of the myosin light chain (MLC) by activating Rhokinase, which is a target of Rho. Several lines of evidence point to an important role of Rho in the action of lysophosphatidic acid (LPA) and thrombin in provoking neurite retraction in N1E-115 neuroblastoma cells. Results: Here we examined whether Rho-kinase and myosin II are involved in neurite retraction in N1E-115 cells. We showed that the expression of constitutively active forms of Rho-kinase induced neurite retraction in N1E-115 cells and MLC phosphorylation in NIH 3T3 cells, whereas the expression of dominant negative forms of Rhokinase inhibited the LPA-induced neurite retraction in N1E-115 cells and the serum-induced MLC phosphorylation in NIH 3T3 cells. The expression of mutant MLC(T18D,S19D) (substitution of Thr and Ser by Asp), which is known to lead to the activation of myosin ATPase and a conformational change of myosin II when reconstituted with myosin heavy chains in vitro, also promoted neurite retraction. Conclusion: These results indicate that Rho-kinase is involved in the LPA-induced neurite retraction downstream of Rho, and that myosin II activation promotes neurite retraction downstream of Rho and Rho-kinase.

本文言語English
ページ(範囲)177-188
ページ数12
ジャーナルGenes to Cells
3
3
DOI
出版ステータスPublished - 24-08-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 細胞生物学

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