Myristoylation-regulated direct interaction between calcium-bound calmodulin and N-terminal region of pp60v-src

Nobuhiro Hayashi, Chisako Nakagawa, Yutaka Ito, Akihiko Takasaki, Yuji Jinbo, Yoshinori Yamakawa, Koiti Titani, Keiichiro Hashimoto, Yoshinobu Izumi, Norio Matsushima

研究成果: Article査読

21 被引用数 (Scopus)

抄録

pp60v-src tyrosine protein kinase was suggested to interact with Ca2+-bound calmodulin (Ca2+/CaM) through the N-terminal region based on its structural similarities to CAP-23/NAP-22, a myristoylated neuron-specific protein, whose myristoyl group is essential for interaction with Ca2+/CaM; (1) the N terminus of pp60 v-src is myristoylated like CAP-23/NAP-22; (2) both lysine residues are required for the myristoylation-dependent interaction and serine residues that are thought to regulate the interaction through the phosphorylations located in the N-terminal region of pp60v-src. To verify this possibility, we investigated the direct interaction between pp60 v-src and Ca2+/CaM using a myristoylated peptide corresponding to the N-terminal region of pp60v-src. The binding assay indicated that only the myristoylated peptide binds to Ca 2+/CaM, and the non-myristoylated peptide is not able to bind to Ca2+/CaM. Analyses of the binding kinetics revealed two independent reactions with the dissociation constants (KD) of 2.07×10 -9M (KD1) and 3.93×10-6M (K D2), respectively. Two serine residues near the myristoyl moiety of the peptide (Ser2, Ser11) were phosphorylated by protein kinase C in vitro, and the phosphorylation drastically reduced the interaction. NMR experiments indicated that two molecules of the myristoylated peptide were bound around the hydrophobic clefts of a Ca2+/CaM molecule. The small-angle X-ray scattering analyses showed that the size of the peptide-Ca2+/CaM complex is 2-3Å smaller than that of the known Ca2+/CaM-target molecule complexes. These results demonstrate clearly the direct interaction between pp60v-src and Ca2+/CaM in a novel manner different from that of known Ca2+/CaM, the target molecules, interactions.

本文言語English
ページ(範囲)169-180
ページ数12
ジャーナルJournal of Molecular Biology
338
1
DOI
出版ステータスPublished - 16-04-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 分子生物学

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