TY - JOUR
T1 - Myt/NZF family transcription factors regulate neuronal differentiation of P19 cells
AU - Kameyama, Toshiki
AU - Matsushita, Fumio
AU - Kadokawa, Yuzo
AU - Marunouchi, Tohru
N1 - Funding Information:
We are grateful to Jun-ichi Miyazaki for providing the pCAGGS expression vector. This work was supported by the “Collaboration with Local Communities” project matching fund subsidy for private universities from the Ministry of Education, Culture, Sports, Science and Technology . T.K. was supported by Grant-in-Aid for Challenging Exploratory Research ( 21657033 ) from the Japan Society for the Promotion of Science (JSPS) .
PY - 2011/6/22
Y1 - 2011/6/22
N2 - During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.
AB - During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.
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U2 - 10.1016/j.neulet.2011.04.033
DO - 10.1016/j.neulet.2011.04.033
M3 - Article
C2 - 21540077
AN - SCOPUS:79956311457
SN - 0304-3940
VL - 497
SP - 74
EP - 79
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -