TY - JOUR
T1 - N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
AU - Murata, Takayuki
AU - Iwahori, Satoko
AU - Okuno, Yusuke
AU - Nishitsuji, Hironori
AU - Yanagi, Yusuke
AU - Watashi, Koichi
AU - Wakita, Takaji
AU - Kimura, Hiroshi
AU - Shimotohno, Kunitada
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/2
Y1 - 2023/2
N2 - N6-methyladenosine (m6A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m6A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m6A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m6A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.
AB - N6-methyladenosine (m6A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m6A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m6A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m6A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.
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U2 - 10.3390/ijms24032265
DO - 10.3390/ijms24032265
M3 - Article
C2 - 36768585
AN - SCOPUS:85147893782
SN - 1661-6596
VL - 24
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 3
M1 - 2265
ER -
