TY - JOUR
T1 - Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells
AU - Ichino, Naohiro
AU - Ishiguro, Hiroshi
AU - Yamada, Kouji
AU - Nishii, Kazuhiro
AU - Sawada, Hirohide
AU - Nagatsu, Toshiharu
N1 - Funding Information:
This work was supported by a grant from the Smoking Research Foundation and a grant-in-aid from Fujita Health University.
PY - 1999/10
Y1 - 1999/10
N2 - The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.
AB - The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.
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U2 - 10.1016/S0168-0102(99)00069-3
DO - 10.1016/S0168-0102(99)00069-3
M3 - Article
C2 - 10555165
AN - SCOPUS:0032828413
SN - 0168-0102
VL - 35
SP - 63
EP - 69
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -