Novel autoimmune phenomena induced in vivo by a new DNA binding protein Nuc: a study on MRL/n mice

Yoshiyuki Kanai, Osamu Takeda, Yukiko Kanai, Keiji Miura, Yoshikazu Kurosawa

研究成果: Article査読

21 被引用数 (Scopus)

抄録

We previously purified a 55 kDa protein that preferentially expands anti-DNA antibody production both in vitro and in vivo across the H-2 barrier from culture supernatants of KML1-7 cells, cloned from a lupus-prone MRL/lpr mouse. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant(r) Nuc in Escherichia coli. To elucidate the function of rNuc in vivo, we initially injected intraperitoneally 5 μg of rNuc without adjuvant into female MRL/n mice at 8 weeks of age and continued injection twice a week. As early as 5 weeks after administration, all mice treated showed an increase in IgG anti-double stranded (ds) DNA antibodies accompanied by IgG hyper-gammaglobulinemia (HG). Of particular interest was that these mice also produced anti-UIRNP antibodies and rheumatoid factor (RF) of IgG class, but not anti-Sm antibodies. Histopathologically, hypercellularity with occasional crescents in the glomeruli was observed, but evidence for lupus nephritis was lacking, indicating that some factors other than Nuc are necessary for the development of a lupus syndrome observed in MRL/lpr mice. Similar administration of lipopolysaccharide into MRL/n mice failed to induce autoantibodies except for a slight increase in serum IgG, suggesting that these autoimmune responses are not due simply to polyclonal B-cell activation. The presence of rNuc will give us a clue for further understanding of autoimmunity.

本文言語English
ページ(範囲)83-89
ページ数7
ジャーナルImmunology Letters
39
1
DOI
出版ステータスPublished - 01-12-1993

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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