Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

Makoto Nakamura, Miya Kobayashi, Takamasa Hanaichi, Koji Hirano, Kunihiko Kobayashi, Takeshi Hoshino, Shinobu Awaya

研究成果: Article査読

7 被引用数 (Scopus)

抄録

We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.

本文言語English
ページ(範囲)185-188
ページ数4
ジャーナルMicroscopy
42
3
DOI
出版ステータスPublished - 06-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 器械工学
  • 放射線学、核医学およびイメージング

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