TY - JOUR
T1 - Odorants suppress voltage-gated currents in retinal horizontal cells in goldfish
AU - Kawai, Fusao
AU - Miyachi, Ei ichi
N1 - Funding Information:
We thank Drs. Michael Freed and Jonathan Demb for critical reading of the manuscript; Drs. Akimichi Kaneko and Takashi Kurahashi for advice. This work was supported by the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan, and Grant-in-Aid for Science Research from the Ministry of Education, Science and Culture (No. 11680794).
PY - 2000/3/10
Y1 - 2000/3/10
N2 - Odorants are known to suppress non-selectively voltage-gated currents in olfactory receptor cells. We found that odorants also suppress voltage-gated currents in neurons of outside of the olfactory system. Under voltage clamp, odorants such as amyl acetate, limonene, and acetophenone suppressed non- selectively voltage-gated currents (a Ca2+ current, a delayed rectifier K+ current, a fast transient K+ current, and an anomalous rectifier K+ current) in horizontal cells from the goldfish retina. An amyl acetate puff completely and immediately suppressed the Ca2+ current (I(Ca)) and the delayed rectifier K+ current induced by repetitive depolarizations, suggesting that amyl acetate is a closed-channel blocker. Odorants did not change significantly the activation curve of I(Ca), but made the slope of inactivation curve of I(Ca) gentler and shifted its half-inactivation voltage toward a negative voltage. These results are similar to the effects of odorants on voltage-gated currents in olfactory receptor cells. This suggests that odorants may suppress the voltage-gated currents in retinal horizontal cells by the same mechanism described in olfactory receptor cells. (C) 2000 Elsevier Science Ireland Ltd.
AB - Odorants are known to suppress non-selectively voltage-gated currents in olfactory receptor cells. We found that odorants also suppress voltage-gated currents in neurons of outside of the olfactory system. Under voltage clamp, odorants such as amyl acetate, limonene, and acetophenone suppressed non- selectively voltage-gated currents (a Ca2+ current, a delayed rectifier K+ current, a fast transient K+ current, and an anomalous rectifier K+ current) in horizontal cells from the goldfish retina. An amyl acetate puff completely and immediately suppressed the Ca2+ current (I(Ca)) and the delayed rectifier K+ current induced by repetitive depolarizations, suggesting that amyl acetate is a closed-channel blocker. Odorants did not change significantly the activation curve of I(Ca), but made the slope of inactivation curve of I(Ca) gentler and shifted its half-inactivation voltage toward a negative voltage. These results are similar to the effects of odorants on voltage-gated currents in olfactory receptor cells. This suggests that odorants may suppress the voltage-gated currents in retinal horizontal cells by the same mechanism described in olfactory receptor cells. (C) 2000 Elsevier Science Ireland Ltd.
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U2 - 10.1016/S0304-3940(00)00822-3
DO - 10.1016/S0304-3940(00)00822-3
M3 - Article
C2 - 10704765
AN - SCOPUS:0034000960
SN - 0304-3940
VL - 281
SP - 151
EP - 154
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2-3
ER -