Okadaic acid enhances prostaglandin E1-induced alkaline phosphatase activity in osteoblast-like cells: Regulation at a point downstream from protein kinase A

Y. Ito, A. Suzuki, Y. Watanabe-Tomita, Y. Oiso, O. Kozawa

研究成果: Article

13 引用 (Scopus)

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We examined the effect of okadaic acid, an inhibitor of protein phosphatase type 1 and 2A, on prostaglandin E1 (PGE1)-induced alkaline phosphatase (ALP) activity in osteoblast-like MC3T3-E1 cells. PGE1 increased ALP activity dose dependently in the range between 10 nM and 0.3 μM in these cells. The pretreatment with okadaic acid enhanced the PGE1-induced ALP activity in a dose-dependent manner in the range between 0.1 and 5 nM. On the other hand, 1-norokadaone, a less potent analogue of okadaic acid, had no effect on the PGE1-induced ALP activity. Tautomycin, an another inhibitor of protein phosphatase type 1 and 2A, also enhanced the PGE1-induced ALP activity. PGE1 stimulated cAMP accumulation dose dependently in the range between 10 nM and 0.3 μM. However, PGE1 had no effect on the formation of inositol phosphates. Okadaic acid did not affect the PGE1-induced cAMP accumulation. Okadaic acid dose dependently enhanced the dibutyryl cAMP-induced ALP activity. These results strongly suggest that protein phosphatase type 1 and/or 2A act as a regulator of ALP activity at a point downstream from protein kinase A in osteoblast-like cells.

元の言語English
ページ(範囲)357-361
ページ数5
ジャーナルProstaglandins Leukotrienes and Essential Fatty Acids
55
発行部数5
DOI
出版物ステータスPublished - 11-1996

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

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