Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification

Daisuke Onoshima; Daiki Kuboyama; Naoto Kihara; Hiromasa Tanaka; Tetsunari Hase; Hiroshi Yukawa; Kenji Ishikawa; Hidefumi Odaka; Yoshinori Hasegawa; Masaru Hori; Yoshinobu Baba

Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification

Daisuke Onoshima
, Daiki Kuboyama
, Naoto Kihara
, Hiromasa Tanaka
, Tetsunari Hase
, Hiroshi Yukawa
, Kenji Ishikawa
, Hidefumi Odaka
, Yoshinori Hasegawa
, Masaru Hori
, Yoshinobu Baba

研究成果: 書籍/レポートタイプへの寄稿 › 会議への寄与

抄録

We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.

本文言語	英語
ホスト出版物のタイトル	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
出版社	Chemical and Biological Microsystems Society
ページ	1291-1293
ページ数	3
ISBN（電子版）	9781510897571
出版ステータス	出版済み - 2018
外部発表	はい
イベント	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, 台湾継続期間: 11-11-2018 → 15-11-2018

出版物シリーズ

名前	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
巻	3

会議

会議	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
国/地域	台湾
City	Kaohsiung
Period	11-11-18 → 15-11-18

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

SDG 3 すべての人に健康と福祉を

All Science Journal Classification (ASJC) codes

化学一般
バイオエンジニアリング
化学工学（その他）
制御およびシステム工学

他のファイルとリンク

引用スタイル

Onoshima, D., Kuboyama, D., Kihara, N., Tanaka, H., Hase, T., Yukawa, H., Ishikawa, K., Odaka, H., Hasegawa, Y., Hori, M., & Baba, Y. (2018). Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1291-1293). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3). Chemical and Biological Microsystems Society.

Onoshima, Daisuke ; Kuboyama, Daiki ; Kihara, Naoto その他. / Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 1291-1293 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

@inproceedings{f2393b96d1c34355a9a2848daa35cfcc,

title = "Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification",

abstract = "We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.",

keywords = "Cancer cell, Liquid biopsy, Membrane separation, Mutation analysis",

author = "Daisuke Onoshima and Daiki Kuboyama and Naoto Kihara and Hiromasa Tanaka and Tetsunari Hase and Hiroshi Yukawa and Kenji Ishikawa and Hidefumi Odaka and Yoshinori Hasegawa and Masaru Hori and Yoshinobu Baba",

note = "Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

language = "English",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

pages = "1291--1293",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

}

Onoshima, D, Kuboyama, D, Kihara, N, Tanaka, H, Hase, T, Yukawa, H, Ishikawa, K, Odaka, H, Hasegawa, Y, Hori, M & Baba, Y 2018, Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 3, Chemical and Biological Microsystems Society, pp. 1291-1293, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, 台湾, 11-11-18.

Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification. / Onoshima, Daisuke; Kuboyama, Daiki; Kihara, Naoto その他.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 1291-1293 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3).

研究成果: 書籍/レポートタイプへの寄稿 › 会議への寄与

TY - GEN

T1 - Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification

AU - Onoshima, Daisuke

AU - Kuboyama, Daiki

AU - Kihara, Naoto

AU - Tanaka, Hiromasa

AU - Hase, Tetsunari

AU - Yukawa, Hiroshi

AU - Ishikawa, Kenji

AU - Odaka, Hidefumi

AU - Hasegawa, Yoshinori

AU - Hori, Masaru

AU - Baba, Yoshinobu

PY - 2018

Y1 - 2018

N2 - We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.

AB - We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.

KW - Cancer cell

KW - Liquid biopsy

KW - Membrane separation

KW - Mutation analysis

UR - https://www.scopus.com/pages/publications/85079786293

UR - https://www.scopus.com/pages/publications/85079786293#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85079786293

T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

SP - 1291

EP - 1293

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

Onoshima D, Kuboyama D, Kihara N, Tanaka H, Hase T, Yukawa H その他. Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 1291-1293. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification

抄録

出版物シリーズ

会議

UN SDG

All Science Journal Classification (ASJC) codes

他のファイルとリンク

フィンガープリント

引用スタイル