TY - JOUR
T1 - Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4
AU - Miura, K.
AU - Hirai, M.
AU - Kanai, Y.
AU - Kurosawa, Y.
N1 - Funding Information:
We thank Ms. K. Nishii, Ms. H. Nomura, and Mr. K. Nishihara for their technical assistance and Ms. A. Suzuoki for preparation of the manuscript. We also thank Dr. A. Awaya for promotion on this work. This work was supported in part by grants from the Ministry of Education, Science and Culture of Japan, the Ministry of Health and Welfare, and Fujita Health University.
PY - 1996
Y1 - 1996
N2 - Nucleobindin (Nuc) was first identified as a secreted protein of 55 kDa that promotes production of DNA-specific antibodies in lupus-prone MRL/lpr mice. Analysis of cDNA that encoded Nuc revealed that the protein is composed of a signal peptide, a DNA-binding site, two calcium-binding motifs (EF-hand motifs), and a leucine zipper. In the present study, we analyzed the organization of the human gene for Nuc (NUC). It consists of 13 exons that are distributed in a region of 32 kb. The functional motifs listed above are encoded in corresponding exons. NUC was expressed in all organs examined. Comparison of nucleotide sequences in the promoter regions between human and mouse NUC genes revealed several conserved sequences. Among them, two Sp1- binding sites and a CCAAT box are of particular interest. The promoter is of the TATA-less type, and transcription starts at multiple sites in both the human and the mouse genes. These features suggest that NUC might normally play a role as a housekeeping gene. NUC was located at human chromosome 19q13.2-q13.4.
AB - Nucleobindin (Nuc) was first identified as a secreted protein of 55 kDa that promotes production of DNA-specific antibodies in lupus-prone MRL/lpr mice. Analysis of cDNA that encoded Nuc revealed that the protein is composed of a signal peptide, a DNA-binding site, two calcium-binding motifs (EF-hand motifs), and a leucine zipper. In the present study, we analyzed the organization of the human gene for Nuc (NUC). It consists of 13 exons that are distributed in a region of 32 kb. The functional motifs listed above are encoded in corresponding exons. NUC was expressed in all organs examined. Comparison of nucleotide sequences in the promoter regions between human and mouse NUC genes revealed several conserved sequences. Among them, two Sp1- binding sites and a CCAAT box are of particular interest. The promoter is of the TATA-less type, and transcription starts at multiple sites in both the human and the mouse genes. These features suggest that NUC might normally play a role as a housekeeping gene. NUC was located at human chromosome 19q13.2-q13.4.
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U2 - 10.1006/geno.1996.0263
DO - 10.1006/geno.1996.0263
M3 - Article
C2 - 8661046
AN - SCOPUS:0029946160
SN - 0888-7543
VL - 34
SP - 181
EP - 186
JO - Genomics
JF - Genomics
IS - 2
ER -