抄録
Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.
| 本文言語 | 英語 |
|---|---|
| ホスト出版物のタイトル | Methods in Molecular Biology |
| 出版社 | Humana Press Inc. |
| ページ | 23-31 |
| ページ数 | 9 |
| DOI | |
| 出版ステータス | 出版済み - 2019 |
| 外部発表 | はい |
出版物シリーズ
| 名前 | Methods in Molecular Biology |
|---|---|
| 巻 | 1576 |
| ISSN（印刷版） | 1064-3745 |
| ISSN（電子版） | 1940-6029 |
All Science Journal Classification (ASJC) codes
- 分子生物学
- 遺伝学
UN SDG
この成果は、次の持続可能な開発目標に貢献しています
