p-aminohippuric acid transport at renal apical membrane mediated by human inorganic phosphate transporter NPT1

Hiroshi Uchino, Ikumi Tamai, Katsumi Yamashita, Yuzuru Minemoto, Yoshimichi Sai, Hikaru Yabuuchi, Ken Ichi Miyamoto, Eiji Takeda, Akira Tsuji

研究成果: Article査読

130 被引用数 (Scopus)

抄録

Organic anions are secreted into urine via organic anion transporters across the renal basolateral and apical membranes. However, no apical membrane transporter for organic anions such as p-aminohippuric acid (PAH) has yet been identified. In the present study, we showed that human NPT1, which is present in renal apical membrane, mediates the transport of PAH. The K(m) value for PAH uptake was 2.66 mM and the uptake was chloride ion sensitive. These results are compatible with those reported for the classical organic anion transport system at the renal apical membrane. PAH transport was inhibited by various anionic compounds. Human NPT1 also accepted uric acid, benzylpenicillin, faropenem, and estradiol-17β-glucuronide as substrates. Considering its chloride ion sensitivity, Npt1 is expected to function for secretion of PAH from renal proximal tubular cells. This is the first molecular demonstration of an organic anion transport function for PAH at the renal apical membrane. (C) 2000 Academic Press.

本文言語English
ページ(範囲)254-259
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
270
1
DOI
出版ステータスPublished - 02-04-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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