Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation

Satoshi Suzuki, Bing Hao, Takafumi Sugawara, Yasushi Matsuda, Toshiharu Tabata, Yasushi Hoshikawa, Yoshifumi Saijo, Takashi Kondo

研究成果: Article

12 引用 (Scopus)

抄録

Background. Cold preservation is the most practical method to maintain the viability of isolated lungs. However, rapid cooling may affect pulmonary endothelial function. We examined the effects of microtubule stabilization with paclitaxel on pulmonary endothelial barrier integrity under cold temperature. Methods. Human pulmonary arterial endothelial cells were incubated at 4°C for 2 hr in the presence or absence of paclitaxel (2.5 μmol/L). Microtubules was visualized using immunocytochemical techniques. Ultrasonic attenuation was measured with scanning acoustic microscopy. Endothelial barrier integrity was measured as transendothelial electric resistance. In addition, we examined graft function in a rat lung transplantation model, in which the donor lung had been preserved in the presence of paclitaxel (2.5 μmol/L) at 4°C for 12 hr. Results. Low temperature caused a reversible microtubule disassembly, but the structure of microtubules was preserved by paclitaxel. Paclitaxel prevented the cooling-induced decrease in ultrasonic attenuation and transendothelial electric resistance. In a rat transplantation model, we found that preservation with paclitaxel successfully improved the oxygenation performance of the donor lung, which demonstrated only mild congestion and less significant interstitial edema without fluid accumulation in the alveolar spaces. Conclusions. Our results indicate that microtubule stabilization with paclitaxel may be beneficial to prevent the loss of the endothelial barrier during cold preservation. We conclude that the use of paclitaxel in organ preservation solutions is useful in protecting pulmonary endothelial barrier integrity during cold preservation, thereby reducing the occurrence of early graft failure.

元の言語English
ページ(範囲)524-529
ページ数6
ジャーナルTransplantation
78
発行部数4
DOI
出版物ステータスPublished - 27-08-2004

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Paclitaxel
Lung
Microtubules
Electric Impedance
Ultrasonics
Organ Preservation Solutions
Acoustic Microscopy
Transplants
Lung Transplantation
Edema
Endothelial Cells
Transplantation
Temperature

All Science Journal Classification (ASJC) codes

  • Transplantation

これを引用

Suzuki, Satoshi ; Hao, Bing ; Sugawara, Takafumi ; Matsuda, Yasushi ; Tabata, Toshiharu ; Hoshikawa, Yasushi ; Saijo, Yoshifumi ; Kondo, Takashi. / Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation. :: Transplantation. 2004 ; 巻 78, 番号 4. pp. 524-529.
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abstract = "Background. Cold preservation is the most practical method to maintain the viability of isolated lungs. However, rapid cooling may affect pulmonary endothelial function. We examined the effects of microtubule stabilization with paclitaxel on pulmonary endothelial barrier integrity under cold temperature. Methods. Human pulmonary arterial endothelial cells were incubated at 4°C for 2 hr in the presence or absence of paclitaxel (2.5 μmol/L). Microtubules was visualized using immunocytochemical techniques. Ultrasonic attenuation was measured with scanning acoustic microscopy. Endothelial barrier integrity was measured as transendothelial electric resistance. In addition, we examined graft function in a rat lung transplantation model, in which the donor lung had been preserved in the presence of paclitaxel (2.5 μmol/L) at 4°C for 12 hr. Results. Low temperature caused a reversible microtubule disassembly, but the structure of microtubules was preserved by paclitaxel. Paclitaxel prevented the cooling-induced decrease in ultrasonic attenuation and transendothelial electric resistance. In a rat transplantation model, we found that preservation with paclitaxel successfully improved the oxygenation performance of the donor lung, which demonstrated only mild congestion and less significant interstitial edema without fluid accumulation in the alveolar spaces. Conclusions. Our results indicate that microtubule stabilization with paclitaxel may be beneficial to prevent the loss of the endothelial barrier during cold preservation. We conclude that the use of paclitaxel in organ preservation solutions is useful in protecting pulmonary endothelial barrier integrity during cold preservation, thereby reducing the occurrence of early graft failure.",
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Suzuki, S, Hao, B, Sugawara, T, Matsuda, Y, Tabata, T, Hoshikawa, Y, Saijo, Y & Kondo, T 2004, 'Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation', Transplantation, 巻. 78, 番号 4, pp. 524-529. https://doi.org/10.1097/01.TP.0000131951.72851.57

Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation. / Suzuki, Satoshi; Hao, Bing; Sugawara, Takafumi; Matsuda, Yasushi; Tabata, Toshiharu; Hoshikawa, Yasushi; Saijo, Yoshifumi; Kondo, Takashi.

:: Transplantation, 巻 78, 番号 4, 27.08.2004, p. 524-529.

研究成果: Article

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AU - Suzuki, Satoshi

AU - Hao, Bing

AU - Sugawara, Takafumi

AU - Matsuda, Yasushi

AU - Tabata, Toshiharu

AU - Hoshikawa, Yasushi

AU - Saijo, Yoshifumi

AU - Kondo, Takashi

PY - 2004/8/27

Y1 - 2004/8/27

N2 - Background. Cold preservation is the most practical method to maintain the viability of isolated lungs. However, rapid cooling may affect pulmonary endothelial function. We examined the effects of microtubule stabilization with paclitaxel on pulmonary endothelial barrier integrity under cold temperature. Methods. Human pulmonary arterial endothelial cells were incubated at 4°C for 2 hr in the presence or absence of paclitaxel (2.5 μmol/L). Microtubules was visualized using immunocytochemical techniques. Ultrasonic attenuation was measured with scanning acoustic microscopy. Endothelial barrier integrity was measured as transendothelial electric resistance. In addition, we examined graft function in a rat lung transplantation model, in which the donor lung had been preserved in the presence of paclitaxel (2.5 μmol/L) at 4°C for 12 hr. Results. Low temperature caused a reversible microtubule disassembly, but the structure of microtubules was preserved by paclitaxel. Paclitaxel prevented the cooling-induced decrease in ultrasonic attenuation and transendothelial electric resistance. In a rat transplantation model, we found that preservation with paclitaxel successfully improved the oxygenation performance of the donor lung, which demonstrated only mild congestion and less significant interstitial edema without fluid accumulation in the alveolar spaces. Conclusions. Our results indicate that microtubule stabilization with paclitaxel may be beneficial to prevent the loss of the endothelial barrier during cold preservation. We conclude that the use of paclitaxel in organ preservation solutions is useful in protecting pulmonary endothelial barrier integrity during cold preservation, thereby reducing the occurrence of early graft failure.

AB - Background. Cold preservation is the most practical method to maintain the viability of isolated lungs. However, rapid cooling may affect pulmonary endothelial function. We examined the effects of microtubule stabilization with paclitaxel on pulmonary endothelial barrier integrity under cold temperature. Methods. Human pulmonary arterial endothelial cells were incubated at 4°C for 2 hr in the presence or absence of paclitaxel (2.5 μmol/L). Microtubules was visualized using immunocytochemical techniques. Ultrasonic attenuation was measured with scanning acoustic microscopy. Endothelial barrier integrity was measured as transendothelial electric resistance. In addition, we examined graft function in a rat lung transplantation model, in which the donor lung had been preserved in the presence of paclitaxel (2.5 μmol/L) at 4°C for 12 hr. Results. Low temperature caused a reversible microtubule disassembly, but the structure of microtubules was preserved by paclitaxel. Paclitaxel prevented the cooling-induced decrease in ultrasonic attenuation and transendothelial electric resistance. In a rat transplantation model, we found that preservation with paclitaxel successfully improved the oxygenation performance of the donor lung, which demonstrated only mild congestion and less significant interstitial edema without fluid accumulation in the alveolar spaces. Conclusions. Our results indicate that microtubule stabilization with paclitaxel may be beneficial to prevent the loss of the endothelial barrier during cold preservation. We conclude that the use of paclitaxel in organ preservation solutions is useful in protecting pulmonary endothelial barrier integrity during cold preservation, thereby reducing the occurrence of early graft failure.

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