Phosphorylation of glial fibrillary, acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase

Hidetaka Kosako, Mutsuki Amano, Maki Yanagida, Kazushi Tanabe, Yoshimi Nishi, Kozo Kaibuchi, Masaki Inagaki

研究成果: Article査読

110 被引用数 (Scopus)

抄録

Site- and phosphorylation state-specific antibodies are useful to analyze spatiotemporal distribution of site-specific phosphorylation of target proteins in vivo. Using several polyclonal and monoclonal antibodies that can specifically recognize four phosphorylated sites on glial fibrillary acidic protein (GFAP), we have previously reported that Thr-7, Ser-13, and Ser-34 on this intermediate filament protein are phosphorylated at the cleavage furrow during cytokinesis. This observation suggests that there exists a protein kinase named cleavage furrow kinase specifically activated at metaphase-anaphase transition (Matsuoka, Y., Nishizawa, K., Yano, T., Shibata, M., Ando, S., Takahashi, T., and Inagaki, M. (1992) EMBO J. 11, 2895-2902; Sekimata, M., Tsujimura, K., Tanaka, J., Takeuchi, Y., Inagaki, N., and Inagaki, M. (1996) J. Cell Biol. 132, 635-641). Here we report that GFAP is phosphorylated specifically at Thr-7, Ser-13, and Ser-34 by Rho-associated kinase (Rho-kinase), which binds to the small GTPase Rho in its GTP-bound active form. The kinase activity of Rho-kinase toward GFAP is dramatically stimulated by guanosine 5′-(3-O-thio)-triphosphate-bound RhoA. Furthermore, the phosphorylation of GFAP by Rho-kinase results in a nearly complete inhibition of its filament formation in vitro, The possibility that Rho-kinase is a candidate for cleavage furrow kinase is discussed.

本文言語English
ページ(範囲)10333-10336
ページ数4
ジャーナルJournal of Biological Chemistry
272
16
DOI
出版ステータスPublished - 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Phosphorylation of glial fibrillary, acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル