Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase

Yasushi Miura; Kozo Kaibuchi; Takahito Itoh; Jackie D. Corbin; Sharron H. Francis; Yoshimi Takai

doi:10.1016/0014-5793(92)80353-I

Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase

Yasushi Miura
, Kozo Kaibuchi
, Takahito Itoh
, Jackie D. Corbin
, Sharron H. Francis
, Yoshimi Takai

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

30 被引用数 (Scopus)

抄録

smg p21B/rap1B p21, a member of ras p21-like small GTP-binding protein superfamily, has been shown to be phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A). We show here that this protein was also phosphorylated by cyclic GMP-dependent protein kinase (protein kinase G) in a cell-free system. The same serine residue (Ser¹⁷⁹) in the C-terminal region was phosphorylated by both protein kinases G and A. The K_m and V_max values of smg p21B for protein kinase G were 5 × 10^-7 M and 4 × 10^-9 mol/min/mg, and those values for protein kinase A were 1 × 10^-7 M and 3 × 10^-8 mol/min/mg.

本文言語	英語
ページ（範囲）	171-174
ページ数	4
ジャーナル	FEBS Letters
巻	297
号	1-2
DOI	https://doi.org/10.1016/0014-5793(92)80353-I
出版ステータス	出版済み - 03-02-1992
外部発表	はい

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

文献へのアクセス

10.1016/0014-5793(92)80353-I

他のファイルとリンク

引用スタイル

@article{b4b796e4d65041e7b4ece4d348c01874,

title = "Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase",

abstract = "smg p21B/rap1B p21, a member of ras p21-like small GTP-binding protein superfamily, has been shown to be phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A). We show here that this protein was also phosphorylated by cyclic GMP-dependent protein kinase (protein kinase G) in a cell-free system. The same serine residue (Ser179) in the C-terminal region was phosphorylated by both protein kinases G and A. The Km and Vmax values of smg p21B for protein kinase G were 5 × 10-7 M and 4 × 10-9 mol/min/mg, and those values for protein kinase A were 1 × 10-7 M and 3 × 10-8 mol/min/mg.",

author = "Yasushi Miura and Kozo Kaibuchi and Takahito Itoh and Corbin, \{Jackie D.\} and Francis, \{Sharron H.\} and Yoshimi Takai",

note = "Funding Information: nclrrro\textasciitilde{}rtlr\textasciitilde{}\textasciitilde{}scrt\textasciitilde{}cr,rs: This investigation at Kobc University was supported by grants-in-aid for Scientific Research and Cancer Research from the Ministry of Education, Science and Culture. Japan (1991), a grant-in-aid for Abnormalities in Hormone Receptor Mechanisms from the Ministry of Health and Welfare, Japan (1991). and by grants from the Human Front\& Science Program (199l), the Yamanouchi Foundation for Research on Metabolic Disease (1991). and the Research Program on Cell Calcium Signal in the Cardiovascular Srstcm (1991). Janan. This invcstination at Vanderbilt University Las supported bi NIH DK40029.-We arc grateful to J. Yamaguchi for her sccretariul assistance.",

year = "1992",

month = feb,

day = "3",

doi = "10.1016/0014-5793(92)80353-I",

language = "English",

volume = "297",

pages = "171--174",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1-2",

}

TY - JOUR

T1 - Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase

AU - Miura, Yasushi

AU - Kaibuchi, Kozo

AU - Itoh, Takahito

AU - Corbin, Jackie D.

AU - Francis, Sharron H.

AU - Takai, Yoshimi

N1 - Funding Information: nclrrro~rtlr~~scrt~cr,rs: This investigation at Kobc University was supported by grants-in-aid for Scientific Research and Cancer Research from the Ministry of Education, Science and Culture. Japan (1991), a grant-in-aid for Abnormalities in Hormone Receptor Mechanisms from the Ministry of Health and Welfare, Japan (1991). and by grants from the Human Front& Science Program (199l), the Yamanouchi Foundation for Research on Metabolic Disease (1991). and the Research Program on Cell Calcium Signal in the Cardiovascular Srstcm (1991). Janan. This invcstination at Vanderbilt University Las supported bi NIH DK40029.-We arc grateful to J. Yamaguchi for her sccretariul assistance.

PY - 1992/2/3

Y1 - 1992/2/3

N2 - smg p21B/rap1B p21, a member of ras p21-like small GTP-binding protein superfamily, has been shown to be phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A). We show here that this protein was also phosphorylated by cyclic GMP-dependent protein kinase (protein kinase G) in a cell-free system. The same serine residue (Ser179) in the C-terminal region was phosphorylated by both protein kinases G and A. The Km and Vmax values of smg p21B for protein kinase G were 5 × 10-7 M and 4 × 10-9 mol/min/mg, and those values for protein kinase A were 1 × 10-7 M and 3 × 10-8 mol/min/mg.

AB - smg p21B/rap1B p21, a member of ras p21-like small GTP-binding protein superfamily, has been shown to be phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A). We show here that this protein was also phosphorylated by cyclic GMP-dependent protein kinase (protein kinase G) in a cell-free system. The same serine residue (Ser179) in the C-terminal region was phosphorylated by both protein kinases G and A. The Km and Vmax values of smg p21B for protein kinase G were 5 × 10-7 M and 4 × 10-9 mol/min/mg, and those values for protein kinase A were 1 × 10-7 M and 3 × 10-8 mol/min/mg.

UR - https://www.scopus.com/pages/publications/0026602309

UR - https://www.scopus.com/pages/publications/0026602309#tab=citedBy

U2 - 10.1016/0014-5793(92)80353-I

DO - 10.1016/0014-5793(92)80353-I

M3 - Article

C2 - 1551424

AN - SCOPUS:0026602309

SN - 0014-5793

VL - 297

SP - 171

EP - 174

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase

抄録

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル