Pituitary adenylate cyclase-activating polypeptide prevents cytokine-induced cytotoxicity via inhibition of inducible nitric oxide synthase expression in βTC cells

Kayo Sekiya, Hiroshi Nagasaki, Nobuaki Ozaki, Atsushi Suzuki, Yoshitaka Miura, Yutaka Oiso

研究成果: Article

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Type 1 diabetes mellitus is an autoimmune disease resulting from apoptotic destruction of pancreatic β-cells. The activation of inducible nitric oxide synthase (iNOS) by inflammatory cytokines is considered a mediator of destruction in β-cells. Recent findings showed that the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP), whose distribution was identified in pancreatic neurons, inhibited nitric oxide (NO) production in cytokine-activated macrophages. In the present study, we investigated the cytoprotective effect of PACAP in the cytokine-exposed mice β-cell line, βTC cells. 1 x 10-8 M PACAP inhibited the reduction of cell viability, NO production, expression of iNOS mRNA, and iNOS promoter activity caused by the combination of three proinflammatory cytokines. Selective iNOS inhibitor also showed the cytoprotective effect in βTC cells. These data suggested that PACAP has a cytoprotective effect in cytokine-treated β-cells through inhibition of iNOS transcription. (C) 2000 Academic Press.

元の言語English
ページ(範囲)211-216
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
278
発行部数1
DOI
出版物ステータスPublished - 11-11-2000
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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