PKCδ inhibition enhances tyrosine hydroxylase phosphorylation in mice after methamphetamine treatment

Eun Joo Shin, Chu Xuan Duong, Xuan Khanh Thi Nguyen, Guoying Bing, Jae Hyung Bach, Dae Hun Park, Keiichi Nakayama, Syed F. Ali, Anumantha G. Kanthasamy, Jean L. Cadet, Toshitaka Nabeshima, Hyoung Chun Kim

研究成果: ジャーナルへの寄稿学術論文査読

36 被引用数 (Scopus)

抄録

The present study was designed to evaluate the specific role of protein kinase C (PKC) δ in methamphetamine (MA)-induced dopaminergic toxicity. A multiple-dose administration regimen of MA significantly increases PKCδ expression, while rottlerin, a PKCδ inhibitor, significantly attenuates MA-induced hyperthermia and behavioral deficits. These behavioral effects were not significantly observed in PKCδ antisense oligonucleotide (ASO)-treated- or PKCδ knockout (-/-)-mice. There were no MA-induced significant decreases of dopamine (DA) content or tyrosine hydroxylase (TH) expression in the striatum in rottlerin-treated-, ASO-treated- or PKCδ (-/-)-mice. The administration of MA also results in a significant decrease of TH phosphorylation at ser 40, but not ser 31, while the inhibition of PKCδ consistently and significantly attenuates MA-induced reduction in the phosphorylation of TH at ser 40. Therefore, these results suggest that the MA-induced enhancement of PKCδ expression is a critical factor in the impairment of TH phosphorylation at ser 40 and that pharmacological or genetic inhibition of PKCδ may be protective against MA-induced dopaminergic neurotoxicity in vivo.

本文言語英語
ページ(範囲)39-50
ページ数12
ジャーナルNeurochemistry International
59
1
DOI
出版ステータス出版済み - 08-2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞および分子神経科学
  • 細胞生物学

フィンガープリント

「PKCδ inhibition enhances tyrosine hydroxylase phosphorylation in mice after methamphetamine treatment」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル