PKN-1, a homologue of mammalian PKN, is involved in the regulation of muscle contraction and force transmission in C. elegans

Hiroshi Qadota, Takayuki Miyauchi, John F. Nahabedian, Jeffrey N. Stirman, Hang Lu, Mutsuki Amano, Guy M. Benian, Kozo Kaibuchi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

To examine the in vivo functions of protein kinase N (PKN), one of the effectors of Rho small guanosine triphosphatases (GTPases), we used the nematode Caenorhabditis elegans as a genetic model system. We identified a C. elegans homologue (pkn-1) of mammalian PKN and confirmed direct binding to C. elegans Rho small GTPases. Using a green fluorescent protein reporter, we showed that pkn-1 is mainly expressed in various muscles and is localized at dense bodies and M lines. Overexpression of the PKN-1 kinase domain and loss-of-function mutations by genomic deletion of pkn-1 resulted in a loopy Unc phenotype, which has been reported in many mutants of neuronal genes. The results of mosaic analysis and body wall muscle-specific expression of the PKN-1 kinase domain suggests that this loopy phenotype is due to the expression of PKN-1 in body wall muscle. The genomic deletion of pkn-1 also showed a defect in force transmission. These results suggest that PKN-1 functions as a regulator of muscle contraction-relaxation and as a component of the force transmission mechanism.

本文言語English
ページ(範囲)222-231
ページ数10
ジャーナルJournal of Molecular Biology
407
2
DOI
出版ステータスPublished - 25-03-2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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