Planar cell polarity induces local microtubule bundling for coordinated ciliary beating

Shogo Nakayama, Tomoki Yano, Toshinori Namba, Satoshi Konishi, Maki Takagishi, Elisa Herawati, Tomoki Nishida, Yasuo Imoto, Shuji Ishihara, Masahide Takahashi, Ken’Ya Furuta, Kazuhiro Oiwa, Atsushi Tamura, Sachiko Tsukita

研究成果: Article査読

抄録

Multiciliated cells (MCCs) in tracheas generate mucociliary clearance through coordinated ciliary beating. Apical microtubules (MTs) play a crucial role in this process by organizing the planar cell polarity (PCP)–dependent orientation of ciliary basal bodies (BBs), for which the underlying molecular basis remains elusive. Herein, we found that the deficiency of Daple, a dishevelled-associating protein, in tracheal MCCs impaired the planar polarized apical MTs without affecting the core PCP proteins, causing significant defects in the BB orientation at the cell level but not the tissue level. Using live-cell imaging and ultra-high voltage electron microscope tomography, we found that the apical MTs accumulated and were stabilized by sideby-side association with one side of the apical junctional complex, to which Daple was localized. In vitro binding and singlemolecule imaging revealed that Daple directly bound to, bundled, and stabilized MTs through its dimerization. These features convey a PCP-related molecular basis for the polarization of apical MTs, which coordinate ciliary beating in tracheal MCCs.

本文言語English
論文番号e202010034
ジャーナルJournal of Cell Biology
220
7
DOI
出版ステータスPublished - 05-07-2021

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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