TY - JOUR
T1 - Plasma jet effectiveness alteration in acute wound healing by binahong (Anredera cordifolia) extract
AU - Darmawati, Sri
AU - Nasruddin, N.
AU - Kurniasiwi, P.
AU - Mukaromah, A. H.
AU - Iswara, A.
AU - Putri, G. S.A.
AU - Rahayu, H. S.E.
AU - Wahyuningtyas, E. S.
AU - Lutfiyati, H.
AU - Kartikadewi, A.
AU - Rejeki, S.
AU - Ishijima, T.
AU - Nakatani, T.
AU - Sugama, J.
N1 - Publisher Copyright:
© 2020 by Begell House, Inc.
PY - 2020
Y1 - 2020
N2 - An atmospheric pressure plasma jet (APPJ) using medical-grade argon gas as the carrier gas was developed and used to investigate wound healing in small animals com-bined with Binahong (Anredera cordifolia) leaf extract treatment. The experiment was divided into 4 treatment groups, control (C), plasma jet (P), Binahong leaf extract (B), and Binahong leaf extract followed by plasma jet (PB). Plasma jet treatment for wounds was applied in different styles based on the treatment day. Both P and PB treatments were contact-style for days 0–4 (5-mm distance for 1-min duration) and noncontact style for days 5–13 (20 mm for 3 min). The total period of wound observation was 14 days. Histological evaluation using hematoxylin-eosin (HE) staining was performed on days 7, 11, and 14 to evaluate wound re-epithelialization. Meanwhile, the number of neutrophil cells was counted using a hematology analyzer on days 11 and 14, and malondialdehyde (MDA) levels were examined on days 7 and 14. This research revealed that plasma jet successfully improved wound healing; on the other hand, plasma jet treatment preceded by Binahong leaf extract treatment tended to impede wound healing. It is hypothesized that Binahong may reduce plasma jet effectiveness in wound healing.
AB - An atmospheric pressure plasma jet (APPJ) using medical-grade argon gas as the carrier gas was developed and used to investigate wound healing in small animals com-bined with Binahong (Anredera cordifolia) leaf extract treatment. The experiment was divided into 4 treatment groups, control (C), plasma jet (P), Binahong leaf extract (B), and Binahong leaf extract followed by plasma jet (PB). Plasma jet treatment for wounds was applied in different styles based on the treatment day. Both P and PB treatments were contact-style for days 0–4 (5-mm distance for 1-min duration) and noncontact style for days 5–13 (20 mm for 3 min). The total period of wound observation was 14 days. Histological evaluation using hematoxylin-eosin (HE) staining was performed on days 7, 11, and 14 to evaluate wound re-epithelialization. Meanwhile, the number of neutrophil cells was counted using a hematology analyzer on days 11 and 14, and malondialdehyde (MDA) levels were examined on days 7 and 14. This research revealed that plasma jet successfully improved wound healing; on the other hand, plasma jet treatment preceded by Binahong leaf extract treatment tended to impede wound healing. It is hypothesized that Binahong may reduce plasma jet effectiveness in wound healing.
UR - https://www.scopus.com/pages/publications/85105343164
UR - https://www.scopus.com/pages/publications/85105343164#tab=citedBy
U2 - 10.1615/PlasmaMed.2021037264
DO - 10.1615/PlasmaMed.2021037264
M3 - Article
AN - SCOPUS:85105343164
SN - 1947-5764
VL - 10
SP - 259
EP - 271
JO - Plasma Medicine
JF - Plasma Medicine
IS - 4
ER -