Possible association between stem-like hallmark and radioresistance in human cervical carcinoma cells

Shoko Kumazawa, Hiroaki Kajiyama, Tomokazu Umezu, Mika Mizuno, Shiro Suzuki, Eiko Yamamoto, Hiroko Mitsui, Ryuichiro Sekiya, Kiyosumi Shibata, Fumitaka Kikkawa

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Aim: We aimed to investigate the possibility of an association between a stem-like hallmark and radiotherapeutic sensitivity in human cervical carcinoma cells. Material and Methods: Side-population (SP) cells and non-SP (NSP) cells in HeLa cells were isolated using flow cytometry and Hoechst 33342 efflux. We performed Western blot analysis to evaluate the expression of stem cell markers (CXCR4, Oct3/4, CD133, and SOX2) and apoptosis markers after irradiation. In addition, SP and NSP cells were injected into nude mice and we assessed subcutaneous tumor formation. To examine tolerance of irradiation, colony formation and apoptosis change were confirmed in the SP and NSP cells. Results: SP cells showed a higher expression of CXCR4, Oct3/4, CD133, and SOX2 than NSP cells. The colony size of SP cells cultured on non-coated dishes was larger than that of NSP cells, and NSP cells were easily induced to undergo apoptosis. SP cells tended to form spheroids and showed a higher level of tumorigenicity compared with NSP cells. In addition, nude mice inoculated with SP cells showed greater tumor growth compared with NSP cells. SP cells showed a higher tumorigenicity and lower apoptotic potential, leading to enhanced radiotolerance. Conclusion: Tumor SP cells showed higher-level stem-cell-like characters and radioresistance than NSP cells. SP cells may be useful for new therapeutic approaches for radiation-resistant cervical cancer.

本文言語English
ページ(範囲)1389-1398
ページ数10
ジャーナルJournal of Obstetrics and Gynaecology Research
40
5
DOI
出版ステータスPublished - 05-2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 産婦人科学

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