Possible effector of Ca24-independent phospholipase a 2, activity in secretory granules from rat parotid gland

M. Mizuno-Kamiya, Y. Kameyama, K. Yashiro, S. -O Shin, J. Fujita

研究成果: Article査読

抄録

To clarify the regulatory mechanism of the Ca2+-independent phospholipase A2 (iPLA2)activity in parotid secretory granules, the effects of various compounds were investigated. The granular iPLA2 activity was not inhibited by either bromoenoJ lactone (BEL) or 5,5′-dithiobis(2-nitrobenzoic acid), which are potent inhibitors of other well-known iPLA2S such as myocardial iPLA2 ATP markedly activated the granular iPLA as well as most iPLAs. Derivatives of ATP (e.g. ATPy S and GTP) were less effective than ATP. Neither addition of K252a (a potent protein kinase inhibitor) nor depletion of Mg2+ affected the ATP-mediated activation. The granular iPLA2 activity was located only in granular membranes, but separation of granular membranes from the granular soluble fraction decreased the activity and ATP-mediated activation of iPLA 2 When the granular soluble fraction was returned to the incubation mixture, both the activity and the ATPmediated activation of iPLAp in the granular membranes were recovered. BSA mimicked the effects of the granular soluble fraction. The addition of free fatty acid reduced the iPLA2 activity. These findings suggest that the granular iPLA2 is regulated through a mechanism mediated directly by ATP. The granular soluble proteins may contribute to the removal of the products from the enzyme protein.

本文言語English
ページ(範囲)A1172
ジャーナルFASEB Journal
11
9
出版ステータスPublished - 01-12-1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

フィンガープリント 「Possible effector of Ca<sub>2</sub>4-independent phospholipase a <sub>2</sub>, activity in secretory granules from rat parotid gland」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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