Possible involvement of protease-activated receptor-1 in the regulation of morphine-induced dopamine release and hyperlocomotion by the tissue plasminogen activator-plasmin system

Mina Ito, Taku Nagai, Hiroyuki Mizoguchi, Ayumi Fukakusa, Yutaka Nakanishi, Hiroyuki Kamei, Toshitaka Nabeshima, Kazuhiro Takuma, Kiyofumi Yamada

研究成果: Article

20 引用 (Scopus)

抄録

We have previously demonstrated that tissue plasminogen activator (tPA)-plasmin system participates in the rewarding effect of morphine, by regulating dopamine release in the nucleus accumbens (NAc). However, it is unclear how plasmin increases the morphine-induced release of dopamine and hyperlocomotion. In the present study we investigated whether protease activated receptor-1 (PAR-1) is involved in the regulation of acute morphine-induced dopamine release by the tPA-plasmin system. Morphine significantly but transiently increased extracellular tPA activity in the NAc, which was completely blocked by naloxone. Microinjection of a PAR-1 antagonist, (tyr -1)-thrombin receptor activating peptide 7, into the NAc significantly reduced morphine-induced dopamine release in the NAc and hyperlocomotion although the treatment had no effect on basal dopamine release and spontaneous locomotor activity. Furthermore, the PAR-1 antagonist blocked the ameliorating effect of plasmin on the defect of morphine-induced dopamine release in the NAc of tPA-deficient mice. In contrast, intracerebroventricular injection of the PAR-1 antagonist had no effect on the antinociceptive effects of morphine in mice. These results suggest that PAR-1 is a target for the tPA-plasmin system in the regulation of acute morphine-induced dopamine release in the NAc.

元の言語English
ページ(範囲)1392-1399
ページ数8
ジャーナルJournal of Neurochemistry
101
発行部数5
DOI
出版物ステータスPublished - 01-06-2007
外部発表Yes

Fingerprint

PAR-1 Receptor
Fibrinolysin
Tissue Plasminogen Activator
Morphine
Dopamine
Nucleus Accumbens
thrombin receptor peptide SFLLRNP
Microinjections
Locomotion
Naloxone
Defects
Injections

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

これを引用

Ito, Mina ; Nagai, Taku ; Mizoguchi, Hiroyuki ; Fukakusa, Ayumi ; Nakanishi, Yutaka ; Kamei, Hiroyuki ; Nabeshima, Toshitaka ; Takuma, Kazuhiro ; Yamada, Kiyofumi. / Possible involvement of protease-activated receptor-1 in the regulation of morphine-induced dopamine release and hyperlocomotion by the tissue plasminogen activator-plasmin system. :: Journal of Neurochemistry. 2007 ; 巻 101, 番号 5. pp. 1392-1399.
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abstract = "We have previously demonstrated that tissue plasminogen activator (tPA)-plasmin system participates in the rewarding effect of morphine, by regulating dopamine release in the nucleus accumbens (NAc). However, it is unclear how plasmin increases the morphine-induced release of dopamine and hyperlocomotion. In the present study we investigated whether protease activated receptor-1 (PAR-1) is involved in the regulation of acute morphine-induced dopamine release by the tPA-plasmin system. Morphine significantly but transiently increased extracellular tPA activity in the NAc, which was completely blocked by naloxone. Microinjection of a PAR-1 antagonist, (tyr -1)-thrombin receptor activating peptide 7, into the NAc significantly reduced morphine-induced dopamine release in the NAc and hyperlocomotion although the treatment had no effect on basal dopamine release and spontaneous locomotor activity. Furthermore, the PAR-1 antagonist blocked the ameliorating effect of plasmin on the defect of morphine-induced dopamine release in the NAc of tPA-deficient mice. In contrast, intracerebroventricular injection of the PAR-1 antagonist had no effect on the antinociceptive effects of morphine in mice. These results suggest that PAR-1 is a target for the tPA-plasmin system in the regulation of acute morphine-induced dopamine release in the NAc.",
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Possible involvement of protease-activated receptor-1 in the regulation of morphine-induced dopamine release and hyperlocomotion by the tissue plasminogen activator-plasmin system. / Ito, Mina; Nagai, Taku; Mizoguchi, Hiroyuki; Fukakusa, Ayumi; Nakanishi, Yutaka; Kamei, Hiroyuki; Nabeshima, Toshitaka; Takuma, Kazuhiro; Yamada, Kiyofumi.

:: Journal of Neurochemistry, 巻 101, 番号 5, 01.06.2007, p. 1392-1399.

研究成果: Article

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