TY - JOUR
T1 - Possible involvement of protein kinase C in interleukin-1 production by mouse peritoneal macrophages
AU - Katakami, Yuko
AU - Nakao, Yoshinobu
AU - Matsui, Toshimitsu
AU - Koizumi, Tamio
AU - Kaibuchi, Kozo
AU - Takai, Yoshimi
AU - Fujita, Takuo
N1 - Funding Information:
*This investigation was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan. 5 To whomc orrespondence should be addressed. 'Abbreviations used are: IL-l, interleukin-1; LPS, lipopolysaccharide; TPA, 12-0-tetradecanoylphorbol-13-acetate; PDBu, phorbol-12,13-dibutyrate; LAL, Limulus amebocyte lysate test; FBS, fetal bovine serum; 2-ME, 2-mercaptoethanol; BSA, bovine serum albumin; PNA-, peanut lectin non-agglutinated; PBS, phosphate-buffered saline; EGTA, ethylene glycol bis[$-aminoethyl ether]- N,N,N',N'-tetraacetic acid; PMSF, phenylmethylsulfonyl fluoride; PI, phosphatidylinositol; [Ca2+]i, cytoplasmic free Ca2+ concentration; PS, phosphatidylserine.
PY - 1986/3/13
Y1 - 1986/3/13
N2 - Both lipopolysaccharide (LPS) and phorbol-12,13-dibutyrate (PDBu), a protein kinase C-activating phorbol ester, induced interleukin-1 (IL-1) production in mouse peritoneal macrophages. Prolonged treatment of the cells with PDBu led to the down-regulation and complete disappearance of protein kinase C. In these cells, PDBu did not increase IL-1 production, but LPS still stimulated IL-1 production although the maximum level was slightly reduced. These results suggest that protein kinase C and another unknown signal pathway are involved in LPS-induced IL-1 production.
AB - Both lipopolysaccharide (LPS) and phorbol-12,13-dibutyrate (PDBu), a protein kinase C-activating phorbol ester, induced interleukin-1 (IL-1) production in mouse peritoneal macrophages. Prolonged treatment of the cells with PDBu led to the down-regulation and complete disappearance of protein kinase C. In these cells, PDBu did not increase IL-1 production, but LPS still stimulated IL-1 production although the maximum level was slightly reduced. These results suggest that protein kinase C and another unknown signal pathway are involved in LPS-induced IL-1 production.
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U2 - 10.1016/0006-291X(86)90002-1
DO - 10.1016/0006-291X(86)90002-1
M3 - Article
C2 - 3083809
AN - SCOPUS:0023047916
SN - 0006-291X
VL - 135
SP - 355
EP - 362
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -