Possible mechanisms underlying the slow lactose fermentation phenotype in Shigella spp.

H. Ito, N. Kido, Y. Arakawa, M. Ohta, T. Sugiyama, N. Kato

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)

抄録

A Southern hybridization analysis revealed that the region homologous to Escherichia coli lacZ was present on the chromosomal DNAs of β-galactosidase-positive Shigella strains, such as Shigella dysenteriae serovar 1 and Shigella sonnei strains, whereas this region was absent from chromosomal DNAs of β-galactosidase-negative strains of Shigella flexneri and Shigella boydii. We found that the lacY-A region was deficient in S. dysenteriae serovar 1 and believe that this is the reason for the slow fermentation of lactose by this strain. S. sonnei strains possessed the region which hybridized with E. coli lacY-A despite their slow hydrolysis of lactose. The whole lactose-fermenting region was cloned from S. sonnei and compared with the cloned lac operon of E. coli K-12. Both clones directed the synthesis of β-galactosidase in an E. coli K-12 strain lacking indigenous β-galactosidase activity (strain JM109-1), and we observed no difference in the expression of β-galactosidase activity in S. sonnei and E. coli. However, E. coli JM109-1 harboring the lactose-fermenting genes of S. sonnei exhibited the slow lactose fermentation phenotype like the parental strain. S. sonnei strains had no detectable lactose permease activities. E. coli JM109-1 harboring the lactose-fermenting genes of S. sonnei had a detectable permease activity, possibly because of the multicopy nature of the cloned genes, but this permease activity was much lower than that of strain JM109-1 harboring the lac operon of E. coli K-12. From these results we concluded that slow lactose fermentation by S. sonnei is due to weak lactose permease activity.

本文言語英語
ページ(範囲)2912-2917
ページ数6
ジャーナルApplied and Environmental Microbiology
57
10
DOI
出版ステータス出版済み - 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー
  • 生態学

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