Preparation of induced pluripotent stem cells using human peripheral blood monocytes

Sumito Isogai, Naoki Yamamoto, Noriko Hiramatsu, Yasuhiro Goto, Masamichi Hayashi, Masashi Kondo, Kazuyoshi Imaizumi

研究成果: Article

抄録

Since induced pluripotent stem (iPS) cells have been established, in recent years, clinical transplantation of cells differentiated from iPS cells derived from human skin fibroblasts is been in progress. On the contrary, monocytes have complete genome information without damage and gene recombination, they are contained in the peripheral blood by ∼3%-8% and differentiate into dendritic cells that are the type of control tower for immune cells. However, generation of monocyte-derived iPS cells has only been successful when special persistent Sendai virus vectors have been used. Therefore, in this study, as a preculture method for monocytes, a culture method for maintaining activity without using any cytokine was established, and using a commercially available vector without genetic toxicity without damaging the chromosome of the cell, iPS cells derived from monocytes were successfully produced. This cell has the ability to differentiate into three germ layers, and when compared with commercially available iPS cells, there was no significant difference between self-renewal and gene expression in the three germ layers. In future, we will compare the differentiation induction of monocyte-derived iPS cells with dendritic cells and investigate the production of dendritic cells that can cope with various antigens.

元の言語English
ページ(範囲)347-355
ページ数9
ジャーナルCellular Reprogramming
20
発行部数6
DOI
出版物ステータスPublished - 01-12-2018

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Induced Pluripotent Stem Cells
Monocytes
Dendritic Cells
Germ Layers
Sendai virus
Genetic Vectors
Cell Transplantation
Genetic Recombination
Fibroblasts
Chromosomes
Genome
Cytokines
Gene Expression
Antigens
Skin
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Developmental Biology
  • Cell Biology

これを引用

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Preparation of induced pluripotent stem cells using human peripheral blood monocytes. / Isogai, Sumito; Yamamoto, Naoki; Hiramatsu, Noriko; Goto, Yasuhiro; Hayashi, Masamichi; Kondo, Masashi; Imaizumi, Kazuyoshi.

:: Cellular Reprogramming, 巻 20, 番号 6, 01.12.2018, p. 347-355.

研究成果: Article

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AU - Yamamoto, Naoki

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AU - Goto, Yasuhiro

AU - Hayashi, Masamichi

AU - Kondo, Masashi

AU - Imaizumi, Kazuyoshi

PY - 2018/12/1

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