Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis

Toru Takano, Takuya Higashiyama, Takashi Uruno, Hiroya Yamada, Hiroshi Yoshida, Akira Miyauchi

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Background. The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. Methods. We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. Results. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases. Conclusion. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.

本文言語English
ページ(範囲)983-990
ページ数8
ジャーナルHead and Neck
30
8
DOI
出版ステータスPublished - 08-2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 耳鼻咽喉科学

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