Preparation of zwitterionic polymethacrylamide modified with L-lysine and its effect on fibrinolytic activity

Zwitterionic polymethacrylamides, poly(N^α-methacrylamide- L-lysine) [poly(α-LysMA)] and poly(N^ε-methacrylamide-L- lysine) [poly(ε-LysMA)] were prepared to examine their bioactivity by an evaluation of fibrinolytic activity and a binding assay using resonant mirror biosensor (IAsys). Poly(α-LysMA) enhanced the fibrinolytic activity by plasminogen/tissue-type plasminogen activator while no enhancement was observed in the case of poly(ε-LysMA). A strong interaction between Plg to poly(α-LysMA) was also observed by IAsys when compared with poly(ε-LysMA).