Presence of spontaneous epithelial-mesenchymal plasticity in esophageal cancer

Kenji Tsuchihashi, Yuki Hirata, Juntaro Yamasaki, Kentaro Suina, Kenro Tanoue, Toshifumi Yae, Kenta Masuda, Eishi Baba, Koichi Akashi, Yuko Kitagawa, Hideyuki Saya, Osamu Nagano

研究成果: ジャーナルへの寄稿学術論文査読

2 被引用数 (Scopus)

抄録

Epithelial-mesenchymal plasticity (EMP) refers to the reversible cellular transition between epithelial and mesenchymal status. Spontaneous EMP is also reported in breast and prostate cancer, leading to the acquisition of stem-cell properties and chemoresistance. However, the presence of spontaneous EMP is still not reported in esophageal cancer. We screened 11 esophageal squamous cancer cell (ESCC) cell lines by CD44 isoform expression. KYSE520 was found to comprise heterogenous populations consisting of CD44v+ and CD44v subpopulations. CD44v+ and CD44v cells showed the expression of epithelial and mesenchymal markers, respectively. Single-cell sorting of CD44v+ and CD44v cells revealed both cells gave rise to cell populations consisting of CD44v+ and CD44v cells, indicating CD44v+ epithelial-like and CD44v mesenchymal-like cells can generate counterparts, respectively. The ablation of Epithelial splicing regulatory protein 1 (ESRP1), a major regulator of CD44 mRNA splicing, resulted in the shift from CD44v+ to CD44v– cells in KYSE520. However, the expression of epithelial-mesenchymal transition (EMT)-related markers or transcriptional factors were almost not affected, suggesting ESRP1 functions downstream of EMP. Our results revealed the presence of spontaneous EMP in esophageal cancer and KYSE520 is useful model to understand spontaneous EMP.

本文言語英語
論文番号101246
ジャーナルBiochemistry and Biophysics Reports
30
DOI
出版ステータス出版済み - 07-2022
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学

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