Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes

Y. Sugimura, T. Murase, K. Oyama, A. Uchida, N. Sato, S. Hayasaka, Y. Kano, Y. Takagishi, Y. Hayashi, Y. Oiso, Y. Murata

研究成果: Article

32 引用 (Scopus)

抄録

Aims/hypothesis: Maternal diabetes during pregnancy increases the risk of congenital malformations such as neural tube defects (NTDs). Although the mechanism of this effect is uncertain, it is known that levels of nitric oxide synthase (NOS) and nitric oxide are elevated in embryos of a mouse model of diabetes. We postulated that overproduction of nitric oxide causes diabetes-induced congenital malformations and that inhibition of inducible NOS (iNOS) might prevent diabetic embryopathy. Methods: Mice were rendered hyperglycaemic by intraperitoneal injection of streptozotocin. The incidence of congenital malformations including NTDs was evaluated on gestational day 18.5. We assessed the involvement of iNOS in diabetes-induced malformation by administering ONO-1714, a specific inhibitor of iNOS, to pregnant mice with streptozotocin-induced diabetic mice and by screening mice with iNOS deficiency due to genetic knockout (iNos -/-). Results: ONO-1714 markedly reduced the incidence of congenital anomalies, including NTDs, in fetuses of a mouse model of diabetes. It also prevented apoptosis in the head region of fetuses, indicating that iNOS is involved in diabetes-related congenital malformations. Indeed, no NTDs were observed in fetuses of diabetic iNos -/- mice and the incidence of other malformations was also markedly reduced. Conclusions/interpretation: We conclude that increased iNOS activity during organogenesis plays a crucial role in the pathogenesis of diabetes-induced malformations and suggest that inhibitors of iNOS might help prevent malformations, especially NTDs, in diabetic pregnancy.

元の言語English
ページ(範囲)962-971
ページ数10
ジャーナルDiabetologia
52
発行部数5
DOI
出版物ステータスPublished - 01-05-2009
外部発表Yes

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Experimental Diabetes Mellitus
Neural Tube Defects
Nitric Oxide Synthase Type II
Fetus
Streptozocin
Incidence
Nitric Oxide
Fetal Diseases
Pregnancy in Diabetics
Organogenesis
Intraperitoneal Injections
Nitric Oxide Synthase
Embryonic Structures
Head
Mothers
Apoptosis
Pregnancy

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

これを引用

Sugimura, Y. ; Murase, T. ; Oyama, K. ; Uchida, A. ; Sato, N. ; Hayasaka, S. ; Kano, Y. ; Takagishi, Y. ; Hayashi, Y. ; Oiso, Y. ; Murata, Y. / Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes. :: Diabetologia. 2009 ; 巻 52, 番号 5. pp. 962-971.
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title = "Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes",
abstract = "Aims/hypothesis: Maternal diabetes during pregnancy increases the risk of congenital malformations such as neural tube defects (NTDs). Although the mechanism of this effect is uncertain, it is known that levels of nitric oxide synthase (NOS) and nitric oxide are elevated in embryos of a mouse model of diabetes. We postulated that overproduction of nitric oxide causes diabetes-induced congenital malformations and that inhibition of inducible NOS (iNOS) might prevent diabetic embryopathy. Methods: Mice were rendered hyperglycaemic by intraperitoneal injection of streptozotocin. The incidence of congenital malformations including NTDs was evaluated on gestational day 18.5. We assessed the involvement of iNOS in diabetes-induced malformation by administering ONO-1714, a specific inhibitor of iNOS, to pregnant mice with streptozotocin-induced diabetic mice and by screening mice with iNOS deficiency due to genetic knockout (iNos -/-). Results: ONO-1714 markedly reduced the incidence of congenital anomalies, including NTDs, in fetuses of a mouse model of diabetes. It also prevented apoptosis in the head region of fetuses, indicating that iNOS is involved in diabetes-related congenital malformations. Indeed, no NTDs were observed in fetuses of diabetic iNos -/- mice and the incidence of other malformations was also markedly reduced. Conclusions/interpretation: We conclude that increased iNOS activity during organogenesis plays a crucial role in the pathogenesis of diabetes-induced malformations and suggest that inhibitors of iNOS might help prevent malformations, especially NTDs, in diabetic pregnancy.",
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Sugimura, Y, Murase, T, Oyama, K, Uchida, A, Sato, N, Hayasaka, S, Kano, Y, Takagishi, Y, Hayashi, Y, Oiso, Y & Murata, Y 2009, 'Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes', Diabetologia, 巻. 52, 番号 5, pp. 962-971. https://doi.org/10.1007/s00125-009-1312-0

Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes. / Sugimura, Y.; Murase, T.; Oyama, K.; Uchida, A.; Sato, N.; Hayasaka, S.; Kano, Y.; Takagishi, Y.; Hayashi, Y.; Oiso, Y.; Murata, Y.

:: Diabetologia, 巻 52, 番号 5, 01.05.2009, p. 962-971.

研究成果: Article

TY - JOUR

T1 - Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes

AU - Sugimura, Y.

AU - Murase, T.

AU - Oyama, K.

AU - Uchida, A.

AU - Sato, N.

AU - Hayasaka, S.

AU - Kano, Y.

AU - Takagishi, Y.

AU - Hayashi, Y.

AU - Oiso, Y.

AU - Murata, Y.

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Aims/hypothesis: Maternal diabetes during pregnancy increases the risk of congenital malformations such as neural tube defects (NTDs). Although the mechanism of this effect is uncertain, it is known that levels of nitric oxide synthase (NOS) and nitric oxide are elevated in embryos of a mouse model of diabetes. We postulated that overproduction of nitric oxide causes diabetes-induced congenital malformations and that inhibition of inducible NOS (iNOS) might prevent diabetic embryopathy. Methods: Mice were rendered hyperglycaemic by intraperitoneal injection of streptozotocin. The incidence of congenital malformations including NTDs was evaluated on gestational day 18.5. We assessed the involvement of iNOS in diabetes-induced malformation by administering ONO-1714, a specific inhibitor of iNOS, to pregnant mice with streptozotocin-induced diabetic mice and by screening mice with iNOS deficiency due to genetic knockout (iNos -/-). Results: ONO-1714 markedly reduced the incidence of congenital anomalies, including NTDs, in fetuses of a mouse model of diabetes. It also prevented apoptosis in the head region of fetuses, indicating that iNOS is involved in diabetes-related congenital malformations. Indeed, no NTDs were observed in fetuses of diabetic iNos -/- mice and the incidence of other malformations was also markedly reduced. Conclusions/interpretation: We conclude that increased iNOS activity during organogenesis plays a crucial role in the pathogenesis of diabetes-induced malformations and suggest that inhibitors of iNOS might help prevent malformations, especially NTDs, in diabetic pregnancy.

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