Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Takayuki Otani, Toshihiro Hashizume, Tadahiro Nagaoka, Tomoko Fukuda, Careen K. Tang, David S. Salomon, Masaharu Seno

研究成果: Article

抄録

The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to approximately 50% of the conventional Fc-fusion design without an Fc domain of IgG. The refolded dimers could be easily analyzed and characterized by SDS-PAGE. Hinge-tagged soluble ErbBs demonstrated significant affinity for betacellulin and heregulin. The IgG hinge-tag should be a simple method to design soluble dimers that would be useful for high throughput screening of ligands, antagonists or derivatives.

元の言語English
ページ(範囲)361-366
ページ数6
ジャーナルBiotechnology Letters
32
発行部数3
DOI
出版物ステータスPublished - 01-02-2010

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Hinges
Immunoglobulin G
Dimers
Neuregulin-1
Disulfides
Polyacrylamide Gel Electrophoresis
Molecular Weight
Ligands
Screening
Fusion reactions
Molecular weight
Throughput
Derivatives
ErbB Receptors
Epidermal Growth Factor

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Otani, Takayuki ; Hashizume, Toshihiro ; Nagaoka, Tadahiro ; Fukuda, Tomoko ; Tang, Careen K. ; Salomon, David S. ; Seno, Masaharu. / Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB). :: Biotechnology Letters. 2010 ; 巻 32, 番号 3. pp. 361-366.
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Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB). / Otani, Takayuki; Hashizume, Toshihiro; Nagaoka, Tadahiro; Fukuda, Tomoko; Tang, Careen K.; Salomon, David S.; Seno, Masaharu.

:: Biotechnology Letters, 巻 32, 番号 3, 01.02.2010, p. 361-366.

研究成果: Article

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