Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry

Kazuki Nakajima, Ayako Kohyama-Koganeya, Yoshio Hirabayashi

研究成果: Chapter

1 被引用数 (Scopus)

抄録

Cell membrane surface glycosphingolipids (GSLs), which are synthesized from glucosylceramide (GlcCer) by the enzymatic action of glucosylceramide synthase (GlcT-1), play fundamental roles in many cellular processes. Recent studies have shown that in adipose tissue, GlcCer is involved in the regulation of energy (fat and sugar) metabolism and that GlcCer acts as a glucose donor in cholesteryl glucoside biosynthesis. To investigate the physiological functions of GlcT-1 and GlcCer, a method to profile GlcCer species by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recently established. The method is based on a combination analysis of retention times in hydrophilic liquid chromatography (HILIC) with specified head group survey, such as neutral-loss and/or precursor-ion scans. Here, the application of this method for profiling GlcCer species in Drosophila melanogaster fat bodies in which Drosophila GLCT-1 (dGlcT-1) expression was manipulated is described, and the implications of these studies in the context of energy metabolism and obesity are discussed.

本文言語English
ホスト出版物のタイトルGlycoscience
ホスト出版物のサブタイトルBiology and Medicine
出版社Springer Japan
ページ565-571
ページ数7
ISBN(電子版)9784431548416
ISBN(印刷版)9784431548409
DOI
出版ステータスPublished - 01-01-2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 医学(全般)

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