Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma

Yuka Muramatsu-Maekawa, Kyojiro Kawakami, Yasunori Fujita, Manabu Takai, Daiki Kato, Keita Nakane, Taku Kato, Tomohiro Tsuchiya, Takuya Koie, Yuri Miura, Masafumi Ito, Kosuke Mizutani

研究成果: ジャーナルへの寄稿学術論文査読

15 被引用数 (Scopus)

抄録

Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC.

本文言語英語
ページ(範囲)253-259
ページ数7
ジャーナルCancer Genomics and Proteomics
18
3
DOI
出版ステータス出版済み - 05-2021
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 遺伝学
  • 癌研究

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