Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds

Ethan A. Englund, Deyun Wang, Hidetsugu Fujigaki, Hiroyasu Sakai, Christopher M. Micklitsch, Rodolfo Ghirlando, Gema Martin-Manso, Michael L. Pendrak, David D. Roberts, Stewart R. Durell, Daniel H. Appella

研究成果: Article査読

74 被引用数 (Scopus)

抄録

Multivalent effects dictate the binding affinity of multiple ligands on one molecular entity to receptors. Integrins are receptors that mediate cell attachment through multivalent binding to peptide sequences within the extracellular matrix, and overexpression promotes the metastasis of some cancers. Multivalent display of integrin antagonists enhances their efficacy, but current scaffolds have limited ranges and precision for the display of ligands. Here we present an approach to studying multivalent effects across wide ranges of ligand number, density, and three-dimensional arrangement. Using L-lysine γ-substituted peptide nucleic acids, the multivalent effects of an integrin antagonist were examined over a range of 1-45 ligands. The optimal construct improves the inhibitory activity of the antagonist by two orders of magnitude against the binding of melanoma cells to the extracellular matrix in both in vitro and in vivo models.

本文言語English
論文番号614
ジャーナルNature communications
3
DOI
出版ステータスPublished - 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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